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In addition, there is no licensed human\n            <jats:named-content content-type=\"genus-species\">S. pyogenes<\/jats:named-content>\n            vaccine. To gain enhanced understanding of the molecular basis of this infection, we employed a multidimensional analysis strategy that included dual RNA-seq and other data derived from experimental infection of nonhuman primates. The data were used to target five streptococcal genes for pathogenesis research, resulting in the unambiguous demonstration that these genes contribute to pathogen-host molecular interactions in necrotizing infections. We exploited fitness data derived from a recently conducted genome-wide transposon mutagenesis study to discover significant correlation between the magnitude of bacterial virulence gene expression\n            <jats:italic>in vivo<\/jats:italic>\n            and pathogen fitness. 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