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Genetic determinants of this newly recognized phenotype are unknown. Previous phenotypic and genotypic characterization of six 2022 Ugandan isolates from UK travelers identified two lines, HL2208 and HL2210, with significantly reduced\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      susceptibility to both artemisinin and lumefantrine and variant alleles of interest at resistance-associated loci. In this study, phenotype-genotype associations are evaluated among an extended panel of 50\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      isolates collected from UK travelers since 2012. Parasites with reduced ring-stage susceptibility to artemisinin\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      carrying newly identified or previously established genetic variants in propeller and\/or non-propeller domains of\n                      <jats:italic toggle=\"yes\">pfk13<\/jats:italic>\n                      came from Kenya, Uganda, Zambia, and Namibia. Variant alleles of\n                      <jats:italic toggle=\"yes\">pfap2\u03bc<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">pfcoronin,<\/jats:italic>\n                      or\n                      <jats:italic toggle=\"yes\">pfubp1<\/jats:italic>\n                      were also identified in some parasite lines. Among 39 evaluable\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      isolates from 15 different countries tested since 2012, six of the ten least susceptible to lumefantrine were from individuals who had traveled to Uganda.\n                      <jats:italic toggle=\"yes\">Pfk13-<\/jats:italic>\n                      dependent and -independent partial artemisinin resistance has arisen in multiple African settings, providing an opportunity for the development of reduced parasite susceptibility to the partner drug lumefantrine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>In our studies of UK travelers returning from Africa with malaria, we have encountered a small number of cases where standard combination drugs have failed to completely clear the infection. In this study, we present new assessments of the effectiveness of the major drugs in use against such parasites and link these findings to the genetic profiles of the parasites causing each infection. We identify new genetic types in some of these patient samples that may provide new markers for monitoring malaria drug resistance in African communities.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mbio.03676-25","type":"journal-article","created":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T14:00:41Z","timestamp":1772028041000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Novel\n                    <i>Pfk13<\/i>\n                    and\n                    <i>Pfubp1<\/i>\n                    genotypes in African\n                    <i>Plasmodium falciparum<\/i>\n                    isolates exhibiting reduced susceptibility to the antimalarials artemisinin and 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