{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:48:58Z","timestamp":1793148538807,"version":"4.5.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973861"],"award-info":[{"award-number":["81973861"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008856","name":"Department of Health of Zhejiang Province","doi-asserted-by":"publisher","award":["20214355173"],"award-info":[{"award-number":["20214355173"]}],"id":[{"id":"10.13039\/501100008856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017529","name":"Major Scientific and Technological Project of Zhejiang Province","doi-asserted-by":"publisher","award":["2023C03044"],"award-info":[{"award-number":["2023C03044"]}],"id":[{"id":"10.13039\/501100017529","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,5,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The correlation between the microbiota found in tumors and tumor development is being progressively understood, specifically regarding its involvement in the initiation and advancement of tumors. We examined a total of 102 samples, examining the microbial composition at the species level in each person unveiled significant variations in both the microbial makeup and tumor proportions among individuals, examining the fluctuating alterations in the microbial profile during breast cancer advancement and progression. The levels of expression for\n              <jats:italic toggle=\"yes\">Pseudoalteromonas elyakovii<\/jats:italic>\n              were notably elevated in the tumor groups when compared to the para-cancer normal group, aligning with the results obtained from qRT-PCR analysis. The relationship between tumor immunity and microorganisms within the tumor was investigated using double immunofluorescence staining combined with SweAMI probe\n              <jats:italic toggle=\"yes\">in situ<\/jats:italic>\n              hybridization and scRNA-seq, allowing for an in-depth analysis of intratumoral microorganisms. Experiments have demonstrated that the supernatant derived from\n              <jats:italic toggle=\"yes\">P. elyakovii<\/jats:italic>\n              displayed a significant ability to promote tumor growth and stimulation. In summary, we describe the characteristics of the intratumoral microbiota and the tumor-promoting effects of\n              <jats:italic toggle=\"yes\">P. elyakovii<\/jats:italic>\n              supernatant within a small dose range in ductal carcinoma of the breast and characterize the potential clinical application value of intratumoural microorganisms in the progression of cancer and immunotherapy.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Despite the existing studies, the specific microbial factors that influence the occurrence and progression of breast cancer still remain unclear. Researchers have clarified the distinctive microbial profile related to ductal carcinoma, a common histological type of breast cancer, in order to identify tumor-specific microbes and their roles in tumorigenesis. With the tumor microbiome as the focus, the enrichment of\n              <jats:italic toggle=\"yes\">Pseudoalteromonas elyakovii<\/jats:italic>\n              features accelerates the disease progression in patients with ductal carcinoma of the breast. This study reveals the initial role relationship and innovative findings between\n              <jats:italic toggle=\"yes\">Pseudoalteromonas elyakovii<\/jats:italic>\n              and ductal carcinoma in the breast.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.03873-24","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T13:05:42Z","timestamp":1744031142000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Tumor-colonizing\n            <i>Pseudoalteromonas elyakovii<\/i>\n            metabolically reprograms the tumor microenvironment and promotes breast ductal carcinoma"],"prefix":"10.1128","volume":"16","author":[{"given":"Shuyan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Breast Surgery, General Surgery, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical 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Breast Surgery, General Surgery, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College","place":["Hangzhou, China"]},{"name":"Key Laboratory for Diagnosis and Treatment of Upper Limb Edema and Stasis of Breast Cancer","place":["Hangzhou, China"]},{"name":"The Second Clinical Medical College, Zhejiang Chinese Medical University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghui","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery, General Surgery, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College","place":["Hangzhou, China"]},{"name":"Key Laboratory for Diagnosis and Treatment of Upper Limb Edema and Stasis of Breast Cancer","place":["Hangzhou, 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Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College","place":["Hangzhou, China"]},{"name":"Key Laboratory for Diagnosis and Treatment of Upper Limb Edema and Stasis of Breast Cancer","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery, General Surgery, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College","place":["Hangzhou, China"]},{"name":"Key Laboratory for Diagnosis and Treatment of Upper Limb Edema and Stasis of Breast Cancer","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiancheng","family":"Mou","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery, General Surgery, Cancer Center, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical 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