{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T18:37:16Z","timestamp":1772735836385,"version":"3.50.1"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["17H05022, 20H02993, 24K08919"],"award-info":[{"award-number":["17H05022, 20H02993, 24K08919"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"G-7 Scholarship Foundation"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mBio"],"published-print":{"date-parts":[[2025,4,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Leaf mold caused by the ascomycete fungus\n              <jats:italic>Cladosporium fulvum<\/jats:italic>\n              is a devastating disease of tomato plants. The mycoparasitic fungus\n              <jats:italic>Hansfordia pulvinata<\/jats:italic>\n              is an effective biocontrol agent that parasitizes\n              <jats:italic>C. fulvum<\/jats:italic>\n              hyphae on leaves and secretes 13-deoxyphomenone, an eremophilane-type sesquiterpene, which was also identified as a sporulation-inducing factor in\n              <jats:italic>Aspergillus oryzae<\/jats:italic>\n              . Here, we identified deoxyphomenone biosynthesis (\n              <jats:italic>DPH<\/jats:italic>\n              ) gene clusters conserved in both\n              <jats:italic>H. pulvinata<\/jats:italic>\n              and\n              <jats:italic>Aspergillus<\/jats:italic>\n              section\n              <jats:italic>Flavi<\/jats:italic>\n              , including\n              <jats:italic>A. oryzae<\/jats:italic>\n              and\n              <jats:italic>A. flavus<\/jats:italic>\n              . Functional disruption of\n              <jats:italic>DPH1<\/jats:italic>\n              orthologous genes encoding sesquiterpene cyclase in\n              <jats:italic>H. pulvinata<\/jats:italic>\n              ,\n              <jats:italic>A. oryzae<\/jats:italic>\n              , and its close relative\n              <jats:italic>A. flavus<\/jats:italic>\n              revealed that deoxyphomenone in\n              <jats:italic>H. pulvinata<\/jats:italic>\n              had exogenic antifungal activity against\n              <jats:italic>C. fulvum<\/jats:italic>\n              and controlled endogenic sporulation in\n              <jats:italic>Aspergillus<\/jats:italic>\n              species. Complete\n              <jats:italic>DPH<\/jats:italic>\n              clusters, highly similar to those in\n              <jats:italic>H. pulvinata<\/jats:italic>\n              , were exclusive to\n              <jats:italic>Aspergillus<\/jats:italic>\n              section\n              <jats:italic>Flavi<\/jats:italic>\n              , while species in other\n              <jats:italic>Aspergillus<\/jats:italic>\n              sections contained fragmented\n              <jats:italic>DPH<\/jats:italic>\n              clusters. A comparative genomics analysis revealed that these\n              <jats:italic>DPH<\/jats:italic>\n              gene clusters share a common origin and are horizontally transferred from an ancestor of\n              <jats:italic>Aspergillus<\/jats:italic>\n              to\n              <jats:italic>H. pulvinata<\/jats:italic>\n              . Our results suggest that after horizontal transfer,\n              <jats:italic>H. pulvinata<\/jats:italic>\n              maintained the\n              <jats:italic>DPH<\/jats:italic>\n              cluster as the inhibitory effect of deoxyphomenone on spore germination and mycelial growth contributed to its mycoparasitism on the host fungus\n              <jats:italic>C. fulvum<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Tomato leaf mold disease caused by\n              <jats:italic>C. fulvum<\/jats:italic>\n              poses a significant economic threat to tomato production globally. Breeders have developed tomato cultivars with\n              <jats:italic>Cf<\/jats:italic>\n              resistance genes.\n              <jats:italic>C. fulvum<\/jats:italic>\n              frequently evolves new races that overcome these genetic defenses, complicating control efforts. Additionally, the pathogen has developed resistance to chemical fungicides, prompting the need for sustainable alternatives like biocontrol agents. The mycoparasitic fungus\n              <jats:italic>H. pulvinata<\/jats:italic>\n              is crucial as an effective agent against\n              <jats:italic>C. fulvum<\/jats:italic>\n              . Clarifying the mechanism of mycoparasitism is significant, as it enhances its application as a biocontrol agent against plant pathogens. This study revealed how\n              <jats:italic>H. pulvinata<\/jats:italic>\n              produces deoxyphomenone, an antifungal compound, through horizontal gene transfer from\n              <jats:italic>Aspergillus<\/jats:italic>\n              species. It is hypothesized that mycoparasitism could be one of the mechanisms that facilitated horizontal gene transfer between fungi. These insights facilitate the development of eco-friendly, sustainable agricultural practices by reducing dependence on chemical fungicides and promoting natural pathogen control methods.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/mbio.04007-24","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T14:33:32Z","timestamp":1742481212000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Adaptive evolution of sesquiterpene deoxyphomenone in mycoparasitism by\n            <i>Hansfordia pulvinata<\/i>\n            associated with horizontal gene transfer from\n            <i>Aspergillus<\/i>\n            species"],"prefix":"10.1128","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9858-0362","authenticated-orcid":false,"given":"Kazuya","family":"Maeda","sequence":"first","affiliation":[{"name":"Laboratory of Plant Pathology, Setsunan University","place":["Hirakata, Japan"]},{"name":"Laboratory of Environmental Microbiology, Setsunan University","place":["Neyagawa, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuya","family":"Sumita","sequence":"additional","affiliation":[{"name":"National Agriculture and Food Research Organization (NARO)","place":["Tsu, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oumi","family":"Nishi","sequence":"additional","affiliation":[{"name":"National Agriculture and Food Research Organization (NARO)","place":["Tsu, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotoshi","family":"Sushida","sequence":"additional","affiliation":[{"name":"National Agriculture and Food Research Organization (NARO)","place":["Tsu, Japan"]},{"name":"Institute of Food Research, National Agriculture and Food Research Organization (NARO)","place":["Tsukuba, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Higashi","sequence":"additional","affiliation":[{"name":"National Agriculture and Food Research Organization (NARO)","place":["Tsu, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Research Center for Advanced Analysis, Core Technology Research Headquarters, National Agriculture and Food Research Organization (NARO)","place":["Tsukuba, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Chemical Biological Sciences, Japan Women\u2019s University","place":["Bunkyo-ku, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eishin","family":"Iwao","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Pathology, Setsunan University","place":["Hirakata, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Much Zaenal","family":"Fanani","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Pathology, Setsunan University","place":["Hirakata, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Nishiya","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Microbiology, Setsunan University","place":["Neyagawa, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5062-3255","authenticated-orcid":true,"given":"Yuichiro","family":"Iida","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Pathology, Setsunan University","place":["Hirakata, Japan"]},{"name":"National Agriculture and Food Research Organization (NARO)","place":["Tsu, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2017.09.003"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13309"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-phyto-011516-040249"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.112408.132637"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123271"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19925"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00999.x"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.12701"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.12916"},{"key":"e_1_3_4_11_2","first-page":"1","article-title":"Studies of micro-fungi XI. Some Hyphomycetes which produce phialides","volume":"45","author":"Hughes SJ","year":"1951","unstructured":"Hughes SJ. 1951. Studies of micro-fungi XI. Some Hyphomycetes which produce phialides. Mycol Papers 45:1\u201336.","journal-title":"Mycol Papers"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1536(72)80140-2"},{"key":"e_1_3_4_13_2","first-page":"316","volume-title":"The genera of fungi sporulating in pure culture","author":"von Arx JA","year":"1981","unstructured":"von Arx JA. 1981. The genera of fungi sporulating in pure culture, p 316\u2013317. \u200e Lubrecht & Cramer Ltd, Port Jervis, NY."},{"key":"e_1_3_4_14_2","first-page":"21","article-title":"The genus Dicyma its synonyms and related fungi","volume":"85","author":"von Arx JA","year":"1982","unstructured":"von Arx JA. 1982. The genus Dicyma its synonyms and related fungi. Proc K Nederl Akad Wet Ser C 85:21\u201328.","journal-title":"Proc K Nederl Akad Wet Ser C"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(83)80051-X"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(01)81717-2"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb1961.48.3189"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53644-9"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.00655-19"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1183"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts386"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2020.10.020"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1304461110"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00263.x"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0075-3"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12362"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/423821"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2016.02.009"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1712798115"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-015-0413-z"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00925-19"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-019-5680-7"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2022.102140"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.15118"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00625309"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-76-990"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-76-1168"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1139\/b87-308"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1982-56762008000100002"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1094\/PHP-2010-0216-01-RS"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007322"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2006.07.052"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0121-1"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.3389\/ffunb.2021.720276"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.05.033"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsz024"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-05-0375"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu033"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac240"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh362"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.14.4427-4435.1993"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.12208"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.919809"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"}],"container-title":["mBio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.04007-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mbio.04007-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T13:04:16Z","timestamp":1744203856000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mbio.04007-24"}},"subtitle":[],"editor":[{"given":"Anuradha","family":"Chowdhary","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,4,9]]},"references-count":55,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4,9]]}},"alternative-id":["10.1128\/mbio.04007-24"],"URL":"https:\/\/doi.org\/10.1128\/mbio.04007-24","relation":{},"ISSN":["2150-7511"],"issn-type":[{"value":"2150-7511","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,9]]},"assertion":[{"value":"2025-01-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04007-24"}}