{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:07:54Z","timestamp":1789477674249,"version":"build-2803163510"},"reference-count":116,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100007699","name":"University of Georgia","doi-asserted-by":"publisher","award":["Start-Up Funds"],"award-info":[{"award-number":["Start-Up Funds"]}],"id":[{"id":"10.13039\/100007699","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Mol Biol Rev"],"abstract":"<jats:title>\n                    <jats:bold>SUMMARY<\/jats:bold>\n                  <\/jats:title>\n                  <jats:p>\n                    Over the past two decades, the field of bioorthogonal chemistry has transitioned from emerging to an established cornerstone of scientific inquiry. In parallel, advances in microbial and host\u2013microbe research have highlighted the need for functional approaches that extend beyond genomic and transcriptomic analyses to directly interrogate protein-level activity. Despite this need, proteomic strategies capable of resolving dynamic, heterogeneous, and low-abundance protein populations remain underdeveloped in microbial systems. This review highlights the convergence of chemo-selective proteomic technologies with microbial biology, focusing on\n                    <jats:underline>b<\/jats:underline>\n                    io\n                    <jats:underline>o<\/jats:underline>\n                    rthogonal\n                    <jats:underline>n<\/jats:underline>\n                    on-\n                    <jats:underline>c<\/jats:underline>\n                    anonical\n                    <jats:underline>a<\/jats:underline>\n                    mino acid\n                    <jats:underline>t<\/jats:underline>\n                    agging (BONCAT), activity- or affinity-based protein profiling, and bioorthogonal post-translational modifications, and comments on possibilities for novel applications for the use of click chemistry-based tools in the functional interrogation of microbial systems. Together, these strategies enable spatiotemporal resolution of protein synthesis, selective profiling of microbial subpopulations, and direct characterization of protein activity and regulation in complex biological contexts, including single-species cultures, host-associated environments, and polymicrobial communities. Continued development and utilization of these technologies will enable deeper mechanistic insight into how microbial systems function and respond to environmental and host-derived cues.\n                  <\/jats:p>","DOI":"10.1128\/mmbr.00099-26","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:35:21Z","timestamp":1789475721000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Chemo-selective proteomics in microbial systems"],"prefix":"10.1128","author":[{"given":"Grace Z.","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05dxps055","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Chemistry and Chemical Engineering, California Institute of Technology","place":["Pasadena, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9139-0443","authenticated-orcid":true,"given":"Chelsey M.","family":"VanDrisse","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02bjhwk41","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Genetics, University of Georgia","place":["Athens, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3451"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1238-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad2602"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.101304.124125"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature19949"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2900"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.01.025"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-018-0183-4"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601637103"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.5c00985"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr5006982"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-021-00028-z"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s44160-021-00017-w"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9007726"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2272-7_14"},{"key":"e_1_3_2_17_2","volume-title":"Cell-selective chemoproteomics for biological discovery","author":"Stone SE","year":"2018","unstructured":"Stone SE. 2018. Cell-selective chemoproteomics for biological discovery. California Institute of Technology."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470559277.ch110148"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja907150m"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(98)00489-x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.012583299"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200602114"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39063-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1216375110"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201002050"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja3004667"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/1439-7633(20020301)3:2\/3<235::AID-CBIC235>3.0.CO;2-7"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200900407"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0177096"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5SC03340C"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1514412113"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007651"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","unstructured":"Wang GZ Warren EA Haas AL Pe\u00f1a AS Kiedrowski MR Lomenick B Chou TF Bomberger JM Tirrell DA Limoli DH. 2023. Staphylococcal secreted cytotoxins are competition sensing signals for Pseudomonas aeruginosa. bioRxiv. doi:10.1101\/2023.01.29.526047","DOI":"10.1101\/2023.01.29.526047"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12436"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16163-2"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02360"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2014.00048"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1603757113"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2023.106679"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00197"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.031914"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00698-y"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.52990"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.09.058"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01628-14"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2016.12.026"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep13430"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1301740111"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1700264114"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1211037"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/416740a"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01593-17"},{"key":"e_1_3_2_53_2","volume-title":"Cell-selective proteomic profiling in complex biological systems","author":"Liu X","year":"2020","unstructured":"Liu X. 2020. Cell-selective proteomic profiling in complex biological systems. California Institute of Technology."},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja400448f"},{"key":"e_1_3_2_55_2","volume-title":"Tools for spatiotemporally specific proteomic analysis in multicellular organisms","author":"Yuet KP","year":"2016","unstructured":"Yuet KP. 2016. Tools for spatiotemporally specific proteomic analysis in multicellular organisms. California Institute of Technology."},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.810180"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1421567112"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2016.05.018"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.11.052"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.054692"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01310-1"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3179"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4201"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature18850"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr2007945"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1223813"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.14694"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio707"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.9b00147"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6439-0_5"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201402582"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470559277.ch090167"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-023-01328-z"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-022-01122-3"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.2c00463"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1039\/d2cb00178k"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2022.108238"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.11.002"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2018.00096"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2017.09.010"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.58"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.07.016"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106781"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2010.196"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12816-z"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2021943118"},{"key":"e_1_3_2_87_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15664"},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1060077"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.50.070181.004031"},{"key":"e_1_3_2_90_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0243-0"},{"key":"e_1_3_2_91_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41570-020-00223-8"},{"key":"e_1_3_2_92_2","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.1.2.207"},{"key":"e_1_3_2_93_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1100"},{"key":"e_1_3_2_94_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13081930"},{"key":"e_1_3_2_95_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-020518-115526"},{"key":"e_1_3_2_96_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00020-15"},{"key":"e_1_3_2_97_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2335201100"},{"key":"e_1_3_2_98_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911247107"},{"key":"e_1_3_2_99_2","doi-asserted-by":"publisher","DOI":"10.1039\/d2cs00764a"},{"key":"e_1_3_2_100_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cws070"},{"key":"e_1_3_2_101_2","doi-asserted-by":"publisher","DOI":"10.1002\/tcr.202100322"},{"key":"e_1_3_2_102_2","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.6b00421"},{"key":"e_1_3_2_103_2","doi-asserted-by":"publisher","DOI":"10.1177\/2472555219887144"},{"key":"e_1_3_2_104_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.101272"},{"key":"e_1_3_2_105_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.352104"},{"key":"e_1_3_2_106_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500042"},{"key":"e_1_3_2_107_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.347260"},{"key":"e_1_3_2_108_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M800187-MCP200"},{"key":"e_1_3_2_109_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr300912q"},{"key":"e_1_3_2_110_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.581843"},{"key":"e_1_3_2_111_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700021-MCP200"},{"key":"e_1_3_2_112_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.101255"},{"key":"e_1_3_2_113_2","doi-asserted-by":"publisher","DOI":"10.1039\/c7sc00247e"},{"key":"e_1_3_2_114_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature25986"},{"key":"e_1_3_2_115_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.07.016"},{"key":"e_1_3_2_116_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-62453-y"},{"key":"e_1_3_2_117_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-59134-1"}],"container-title":["Microbiology and Molecular Biology Reviews"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mmbr.00099-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mmbr.00099-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:35:23Z","timestamp":1789475723000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mmbr.00099-26"}},"subtitle":[],"editor":[{"given":"Corrella S.","family":"Detweiler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":116,"alternative-id":["10.1128\/mmbr.00099-26"],"URL":"https:\/\/doi.org\/10.1128\/mmbr.00099-26","relation":{},"ISSN":["1092-2172","1098-5557"],"issn-type":[{"value":"1092-2172","type":"print"},{"value":"1098-5557","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"2026-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00099-26"}}