{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:39:46Z","timestamp":1792510786063,"version":"4.5.0"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["7005333"],"award-info":[{"award-number":["7005333"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01DK147125"],"award-info":[{"award-number":["R01DK147125"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Resour Announc"],"published-print":{"date-parts":[[2026,10,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:bold>\n                        <jats:underline>Meta<\/jats:underline>\n                      <\/jats:bold>\n                      genomic\n                      <jats:bold>\n                        <jats:underline>C<\/jats:underline>\n                      <\/jats:bold>\n                      overage and\n                      <jats:bold>\n                        <jats:underline>D<\/jats:underline>\n                      <\/jats:bold>\n                      epth Calculator (Meta-CD) is a convenient, biologist-friendly tool for determining coverage and depth to enhance taxonomic detection, functional profiling, and metagenome-assembled genome (MAG) recovery in metagenomics. It supports experimental design and post-sequencing analysis, modeling how genome size, relative abundance, sequencing depth, and DNA quantity influence detection of target species.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/mra.00811-26","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:41:25Z","timestamp":1788529285000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Meta-CD: a metagenomic sequencing coverage and depth calculator for target species"],"prefix":"10.1128","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4215-8535","authenticated-orcid":true,"given":"Callie","family":"Claiborne","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6b6f76","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant and Microbial Biology, North Carolina State University","place":["Raleigh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0184-6609","authenticated-orcid":true,"given":"Zhe","family":"Lyu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b6b6f76","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant and Microbial Biology, North Carolina State University","place":["Raleigh, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-015-0611-7"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-026-02334-2"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.007"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2375"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbac443"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-017-0012-7"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(88)90007-9"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2020.02.003"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2019.0264"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sdata.2016.81"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00285-012-0586-x"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3103"}],"container-title":["Microbiology Resource Announcements"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mra.00811-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mra.00811-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:50:32Z","timestamp":1792507832000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mra.00811-26"}},"subtitle":[],"editor":[{"given":"Patrick D.","family":"Schloss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,10,8]]},"references-count":12,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,8]]}},"alternative-id":["10.1128\/mra.00811-26"],"URL":"https:\/\/doi.org\/10.1128\/mra.00811-26","relation":{},"ISSN":["2576-098X"],"issn-type":[{"value":"2576-098X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,8]]},"assertion":[{"value":"2026-06-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00811-26"}}