{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:23:12Z","timestamp":1753881792735,"version":"3.41.2"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"European Fund for Regional Development","award":["BG05M2OP001-1.002-0001-C04"],"award-info":[{"award-number":["BG05M2OP001-1.002-0001-C04"]}]},{"DOI":"10.13039\/501100003336","name":"Bulgarian National Science Fund","doi-asserted-by":"publisher","award":["DN 13\/4-15.12.2017"],"award-info":[{"award-number":["DN 13\/4-15.12.2017"]}],"id":[{"id":"10.13039\/501100003336","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Resour Announc"],"published-print":{"date-parts":[[2021,1,21]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">Mycobacterium bovis<\/jats:named-content>\n            BCG SL222 Sofia is a substrain descending from the Russian BCG-I vaccine strain. Here, we report the complete genome sequence of BCG SL222 Sofia, which will facilitate identity assurance and will contribute to more consistent manufacturing, standardization, and differentiation of substrains used in vaccine production.\n          <\/jats:p>","DOI":"10.1128\/mra.01318-20","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T13:39:59Z","timestamp":1611149999000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Complete Genome Sequence of Mycobacterium bovis BCG SL222 Sofia, the First WHO Reference Reagent for the M. bovis BCG Vaccine of the Russian BCG-I Substrain"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9997-5198","authenticated-orcid":false,"given":"Stefan","family":"Panaiotov","sequence":"first","affiliation":[{"name":"National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yordan","family":"Hodzhev","sequence":"additional","affiliation":[{"name":"National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Tolchkov","sequence":"additional","affiliation":[{"name":"National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Mihailov","sequence":"additional","affiliation":[{"name":"BB-NCIPD Ltd., Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roumen","family":"Kofinov","sequence":"additional","affiliation":[{"name":"BB-NCIPD Ltd., Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todor","family":"Kantardjiev","sequence":"additional","affiliation":[{"name":"National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzvetelina","family":"Stefanova","sequence":"additional","affiliation":[{"name":"BB-NCIPD Ltd., Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.10.066"},{"key":"e_1_3_2_3_2","volume-title":"International collaborative study to evaluate and establish the 1st WHO reference reagents for BCG vaccines of Russian BCG-I sub-strain.","author":"Markey K","year":"2010","unstructured":"Markey K, Rigsby P, Hockley J, Ho MM. 2010. International collaborative study to evaluate and establish the 1st WHO reference reagents for BCG vaccines of Russian BCG-I sub-strain. Publication WHO\/BS\/10.2148. World Health Organization, Geneva, Switzerland. https:\/\/apps.who.int\/iris\/handle\/10665\/70582."},{"key":"e_1_3_2_4_2","volume-title":"WHO reference reagent: the 1st WHO reference reagent for BCG vaccine of Russian BCG-I sub-strain: NIBSC code 07\/274","author":"National Institute for Biological Standards and Control","year":"2013","unstructured":"National Institute for Biological Standards and Control. 2013. WHO reference reagent: the 1st WHO reference reagent for BCG vaccine of Russian BCG-I sub-strain: NIBSC code 07\/274, version 3.0. World Health Organization, Geneva, Switzerland. https:\/\/www.nibsc.org\/documents\/ifu\/07-274.pdf.","edition":"3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00182-16"},{"key":"e_1_3_2_6_2","first-page":"49","article-title":"Identity and stability assessment of BCG vaccine by multiplex PCR","volume":"16","author":"Levi DT","year":"2016","unstructured":"Levi DT, Obukhov YI, Aleksandrova NV, Volkova RA, Elbert EV, Alvarez Figeroa MV, Prokopenko AV, Ludanniy RI. 2016. Identity and stability assessment of BCG vaccine by multiplex PCR. Bioprep Prevent Diagn Treat 16:49\u201354. (In Russian.)","journal-title":"Bioprep Prevent Diagn Treat"},{"key":"e_1_3_2_7_2","first-page":"28","volume-title":"WHO technical report series 745.","author":"WHO Expert Committee on Biological Standardization","year":"1987","unstructured":"WHO Expert Committee on Biological Standardization. 1987. Requirements for dried BCG vaccine, p 28. In WHO technical report series 745. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_8_2","first-page":"35","volume-title":"WHO technical report series 771.","author":"WHO Expert Committee on Biological Standardization","year":"1988","unstructured":"WHO Expert Committee on Biological Standardization. 1988. Requirements for dried BCG vaccine (amendment 1987), p 35. In WHO technical report series 771. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_9_2","first-page":"183","article-title":"Le BCG et la prevention de la tuberculose","volume":"27","author":"Gu\u00e9rin C","year":"1948","unstructured":"Gu\u00e9rin C. 1948. Le BCG et la prevention de la tuberculose. Rev Atomes 27:183\u2013188.","journal-title":"Rev Atomes"},{"key":"e_1_3_2_10_2","first-page":"347","article-title":"BCG on Sauton's medium: effect of a long series of subcultures on the morphological and biological properties of BCG cultures","volume":"2","author":"Van Deinse F","year":"1950","unstructured":"Van Deinse F, Senechal F. 1950. BCG on Sauton's medium: effect of a long series of subcultures on the morphological and biological properties of BCG cultures. Bull World Health Organ 2:347\u2013354.","journal-title":"Bull World Health Organ"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)35141-4"},{"key":"e_1_3_2_12_2","volume-title":"MiSeq reporter software guide.","author":"Illumina","year":"2017","unstructured":"Illumina. 2017. MiSeq reporter software guide. Illumina proprietary document 15042295 v05. Illumina, San Diego, CA. https:\/\/support.illumina.com\/content\/dam\/illumina-support\/documents\/documentation\/software_documentation\/miseqreporter\/miseq-reporter-software-guide-15042295-05.pdf."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr026"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr507"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts199"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw569"}],"container-title":["Microbiology Resource Announcements"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/MRA.01318-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/MRA.01318-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T05:51:46Z","timestamp":1646718706000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/MRA.01318-20"}},"subtitle":[],"editor":[{"given":"Julie C.","family":"Dunning Hotopp","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,1,21]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,1,21]]}},"alternative-id":["10.1128\/MRA.01318-20"],"URL":"https:\/\/doi.org\/10.1128\/mra.01318-20","relation":{},"ISSN":["2576-098X"],"issn-type":[{"type":"electronic","value":"2576-098X"}],"subject":[],"published":{"date-parts":[[2021,1,21]]},"assertion":[{"value":"2020-11-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01318-20"}}