{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:38:57Z","timestamp":1790419137726,"version":"4.1.0"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T00:00:00Z","timestamp":1622678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T00:00:00Z","timestamp":1622678400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Resour Announc"],"published-print":{"date-parts":[[2021,6,3]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">Clostridium botulinum<\/jats:named-content>\n            group III is the anaerobic Gram-positive bacteria producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from\n            <jats:named-content content-type=\"genus-species\">Clostridium botulinum<\/jats:named-content>\n            group III neurotoxin types C, D, C\/D, and D\/C. The protocol to obtain high-molecular-weight DNA from\n            <jats:named-content content-type=\"genus-species\">C. botulinum<\/jats:named-content>\n            group III is described.\n          <\/jats:p>","DOI":"10.1128\/mra.01364-20","type":"journal-article","created":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T02:09:35Z","timestamp":1623377375000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3673-6082","authenticated-orcid":true,"given":"Cedric","family":"Woudstra","sequence":"first","affiliation":[{"name":"Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommi","family":"M\u00e4klin","sequence":"additional","affiliation":[{"name":"Department of Mathematics and Statistics, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yagmur","family":"Derman","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Bano","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Istituto Zooprofiilattico Sperimentale delle Venezie, Villorba, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Skarin","sequence":"additional","affiliation":[{"name":"National Veterinary Institute, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Mazuet","sequence":"additional","affiliation":[{"name":"National Reference Center for Anaerobic Bacteria and Botulism, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antti","family":"Honkela","sequence":"additional","affiliation":[{"name":"Department of Computer Science, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miia","family":"Lindstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Environmental Health, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/9781118728291.ch26"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1024\/0036-7281.148.7.331"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2014.957644"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2015.09.007"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107777"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2010.526923"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505503102"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039107"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1121546109"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky370"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr039"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw255"}],"container-title":["Microbiology Resource Announcements"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/MRA.01364-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/MRA.01364-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T08:18:11Z","timestamp":1646727491000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/MRA.01364-20"}},"subtitle":[],"editor":[{"given":"Irene L. G.","family":"Newton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,6,3]]},"references-count":12,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2021,6,3]]}},"alternative-id":["10.1128\/MRA.01364-20"],"URL":"https:\/\/doi.org\/10.1128\/mra.01364-20","relation":{},"ISSN":["2576-098X"],"issn-type":[{"value":"2576-098X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,3]]},"assertion":[{"value":"2021-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01364-20"}}