{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:06:29Z","timestamp":1775055989197,"version":"3.50.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T00:00:00Z","timestamp":1625011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T00:00:00Z","timestamp":1625011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19fk0108150, JP20fk0108150"],"award-info":[{"award-number":["JP19fk0108150, JP20fk0108150"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2021,6,30]]},"abstract":"<jats:p>A growing number of studies suggest the potential role of asymptomatic SARS-CoV-2 carriers as a major driver of the COVID-19 pandemic; however, virological assessment of asymptomatic infection has largely been limited to reverse transcription-PCR (RT-PCR), which can be persistently positive without necessarily indicating the presence of viable virus (e.g., replication-competent virus). Here, we evaluated the infectivity of asymptomatic SARS-CoV-2 carriers by detecting SARS-CoV-2-induced cytopathic effects on Vero cells using longitudinally obtained nasopharyngeal samples from asymptomatic carriers.<\/jats:p>","DOI":"10.1128\/msphere.00019-21","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T13:04:06Z","timestamp":1621947846000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Shedding of Viable Virus in Asymptomatic SARS-CoV-2 Carriers"],"prefix":"10.1128","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7228-0839","authenticated-orcid":true,"given":"Takayuki","family":"Murata","sequence":"first","affiliation":[{"name":"Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, 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