{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T02:51:07Z","timestamp":1774061467548,"version":"3.50.1"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T00:00:00Z","timestamp":1461715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T00:00:00Z","timestamp":1461715200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["2R15GM104880"],"award-info":[{"award-number":["2R15GM104880"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R15GM104880-01A1"],"award-info":[{"award-number":["R15GM104880-01A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1263905"],"award-info":[{"award-number":["1263905"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2016,4,27]]},"abstract":"<jats:p>\n            Glycine is required for various cellular functions, including cell wall synthesis, protein synthesis, and the biosynthesis of several important metabolites. Regulating levels of glycine metabolism allows\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            to maintain the metabolic flux of glycine through several pathways, including the metabolism of glycine to produce other amino acids, entry into the trichloroacetic acid cycle, and the production of virulence factors such as hydrogen cyanide. In this study, we characterized GcsR, a transcriptional regulator that activates the expression of genes involved in\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            PAO1 glycine metabolism. Our work reveals that GcsR is the founding member of a novel class of TyrR-like EBPs that likely regulate glycine metabolism in\n            <jats:italic>Pseudomonadales<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/msphere.00020-16","type":"journal-article","created":{"date-parts":[[2016,4,28]],"date-time":"2016-04-28T03:32:53Z","timestamp":1461814373000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["GcsR, a TyrR-Like Enhancer-Binding Protein, Regulates Expression of the Glycine Cleavage System in Pseudomonas aeruginosa PAO1"],"prefix":"10.1128","volume":"1","author":[{"given":"Zaara","family":"Sarwar","sequence":"first","affiliation":[{"name":"Department of Chemistry, State University of New York\u2014College of Environmental Science and Forestry, Syracuse, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin R.","family":"Lundgren","sequence":"additional","affiliation":[{"name":"Department of Chemistry, State University of New York\u2014College of Environmental Science and Forestry, Syracuse, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael T.","family":"Grassa","sequence":"additional","affiliation":[{"name":"Department of Chemistry, State University of New York\u2014College of Environmental Science and Forestry, Syracuse, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael X.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, State University of New York\u2014College of Environmental Science and Forestry, Syracuse, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan","family":"Gribble","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer F.","family":"Moffat","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6981-5679","authenticated-orcid":false,"given":"Christopher T.","family":"Nomura","sequence":"additional","affiliation":[{"name":"Department of Chemistry, State University of New York\u2014College of Environmental Science and Forestry, Syracuse, New York, USA"},{"name":"Center for Applied Microbiology, State University of New York\u2014College of Environmental Science and Forestry, Syracuse, New York, USA"},{"name":"Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.97.1.282-291.1969"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.100.1.276-282.1969"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(51)90240-8"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01659328"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)93558-1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.130.2.826-831.1977"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.84.246"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0610315"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.163.1.1-7.1985"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb17578.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.9.5095-5102.1989"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02205-12"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00006-12"},{"key":"e_1_3_2_15_2","first-page":"529","article-title":"Regulation of aromatic amino acid biosynthesis in gamma-proteobacteria","volume":"3","author":"Panina EM","year":"2001","unstructured":"Panina EM Vitreschak AG Mironov AA Gelfand MS . 2001. Regulation of aromatic amino acid biosynthesis in gamma-proteobacteria. J Mol Microbiol Biotechnol3:529\u2013543.","journal-title":"J Mol Microbiol Biotechnol"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04385.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.00131.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02124.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq869"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00112-10"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01050-13"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/006247-0"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.17.4940-4946.1995"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-7-2203"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01615-14"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.4.1053-1061.2000"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.8.4360"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1490"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.24.13904"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024310"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.18.5213-5222.2001"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.10.2702-2708.2000"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.15202"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.21.6207-6214.2001"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02124.x"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.12.018"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.8.4326-4333.1989"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01055-13"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01393-07"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(76)90173-2"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002039900127"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2009.12.003"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2004.10.002"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-013-1552-5"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00526-12"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-08-13-0219-R"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.090166"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2012.10.004"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2036282100"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09367.x"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.4.1246-1253.2005"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01515-09"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05074-11"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00225-12"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-30"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00129-15"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt942"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00338-07"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000163"},{"key":"e_1_3_2_60_2","first-page":"1","volume-title":"The nematode Caenorhabditis elegans","author":"Wood WB","year":"1988","unstructured":"Wood WB . 1988. Introduction to C. elegans biology, p 1\u201316. In WoodWB (ed), The nematode Caenorhabditis elegans. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY."},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(76)90119-6"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.7.2227-2235.2003"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2007.01380.x"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/msb4100050"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00584-1"}],"updated-by":[{"DOI":"10.1128\/msphere.00200-16","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T00:00:00Z","timestamp":1469577600000}}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00020-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00020-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T03:46:15Z","timestamp":1646711175000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00020-16"}},"subtitle":[],"editor":[{"given":"Karen L.","family":"Visick","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,4,27]]},"references-count":64,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,4,27]]}},"alternative-id":["10.1128\/mSphere.00020-16"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00020-16","relation":{"correction":[{"id-type":"doi","id":"10.1128\/mSphere.00200-16","asserted-by":"object"}]},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,27]]},"assertion":[{"value":"2016-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00020-16"}}