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The pathogenesis of\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            is linked to its lytic cycle, which starts when tachyzoites invade host cells and secrete proteins from specialized organelles. Once inside the host cell, the parasite creates a parasitophorous vacuole (PV) where it divides. Rhoptries are specialized secretory organelles that contain proteins, many of which are secreted during invasion. These proteins have important roles not only during the initial interaction between parasite and host but also in the formation of the PV and in the modification of the host cell. We report here the identification of a new\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            carbonic anhydrase-related protein (TgCA_RP), which localizes to rhoptries of mature tachyzoites. TgCA_RP is important for the morphology of rhoptries and for invasion and growth of parasites. TgCA_RP is also critical for parasite virulence. We propose that TgCA_RP plays a role in the biogenesis of rhoptries.\n          <\/jats:p>","DOI":"10.1128\/msphere.00027-17","type":"journal-article","created":{"date-parts":[[2017,5,18]],"date-time":"2017-05-18T00:30:43Z","timestamp":1495067443000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["A Glycosylphosphatidylinositol-Anchored Carbonic Anhydrase-Related Protein of Toxoplasma gondii Is Important for Rhoptry Biogenesis and Virulence"],"prefix":"10.1128","volume":"2","author":[{"given":"Nathan M.","family":"Chasen","sequence":"first","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, USA"},{"name":"Department of Infectious Diseases, University of Georgia, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beejan","family":"Asady","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro","family":"Lemgruber","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Metrologia, Inmetro-RJ, UFRJ, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossiane C.","family":"Vommaro","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica C.","family":"Kissinger","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, USA"},{"name":"Department of Genetics, University of Georgia, Athens, Georgia, USA"},{"name":"Institute of Bioinformatics, University of Georgia, Athens, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Coppens","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia N. 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