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CVB is transmitted via the fecal-oral route and encounters the epithelium lining the gastrointestinal tract early in infection. The lack of suitable\n            <jats:italic>in vivo<\/jats:italic>\n            and\n            <jats:italic>in vitro<\/jats:italic>\n            models to study CVB infection of the gastrointestinal epithelium has limited our understanding of the events that surround infection of these specialized cells. Here, we report on the development of a three-dimensional (3-D) organotypic cell culture model of human intestinal epithelial cells that better models the gastrointestinal epithelium\n            <jats:italic>in vivo<\/jats:italic>\n            . By applying this 3-D model, which recapitulates many aspects of the gastrointestinal epithelium\n            <jats:italic>in vivo<\/jats:italic>\n            , to the study of CVB infection, our work provides a new cell system to model the mechanisms by which CVB infects the intestinal epithelium, which may have a profound impact on CVB pathogenesis.\n          <\/jats:p>","DOI":"10.1128\/msphere.00030-15","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T17:49:26Z","timestamp":1447782566000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["A Three-Dimensional Cell Culture Model To Study Enterovirus Infection of Polarized Intestinal Epithelial Cells"],"prefix":"10.1128","volume":"1","author":[{"given":"Coyne G.","family":"Drummond","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9804-9739","authenticated-orcid":false,"given":"Cheryl A.","family":"Nickerson","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona, USA"},{"name":"The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolyn B.","family":"Coyne","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90690-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.3.1903-1906.1995"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90168-E"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02675-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/175.3.697"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.18.9474-9480.2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03468-14"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.94.1.45"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.96.5.491"},{"key":"e_1_3_2_11_2","first-page":"241","article-title":"Myocarditis induced by coxsackie B3 virus in mature mice","volume":"23","author":"Jaskiewicz K","year":"1975","unstructured":"Jaskiewicz K Mrozinska B . 1975. 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