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The recent analysis of the\n            <jats:italic>Toxoplasma<\/jats:italic>\n            \u201cubiquitome\u201d highlights the importance of ubiquitination in the parasite cell cycle. The machinery regulating the ubiquitin dynamics in\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            has remained understudied. Here, we provide a biochemical characterization of an OTU (\n            <jats:italic>o<\/jats:italic>\n            varian\n            <jats:italic>tu<\/jats:italic>\n            mor) family deubiquitinase, TgOTUD3A, defining its localization and dynamic expression pattern at various stages of the cell cycle. We further establish that TgOTUD3A has activity preference for polyubiquitin chains with certain lysine linkages\u2014such unique activity has not been previously reported in any apicomplexan. This is particularly important given the finding in this study that\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            proteins are modified by diverse lysine-linked polyubiquitin chains and that these modifications are very dynamic across the cell cycle, pointing toward the sophistication of the \u201cubiquitin code\u201d as a potential mechanism to regulate parasite biology.\n          <\/jats:p>","DOI":"10.1128\/msphere.00085-16","type":"journal-article","created":{"date-parts":[[2016,6,23]],"date-time":"2016-06-23T02:44:06Z","timestamp":1466649846000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["A Cell Cycle-Regulated\n            <i>Toxoplasma<\/i>\n            Deubiquitinase, TgOTUD3A, Targets Polyubiquitins with Specific Lysine Linkages"],"prefix":"10.1128","volume":"1","author":[{"given":"Animesh","family":"Dhara","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony P.","family":"Sinai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.03.007"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1079\/AHR2005100"},{"key":"e_1_3_2_4_2","first-page":"414","article-title":"Prevalence of Toxoplasma infection in a cohort of homosexual men at risk of AIDS and toxoplasmic encephalitis","volume":"6","author":"Israelski DM","year":"1993","unstructured":"Israelski DM Chmiel JS Poggensee L Phair JP Remington JS . 1993. Prevalence of Toxoplasma infection in a cohort of homosexual men at risk of AIDS and toxoplasmic encephalitis. 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