{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:43:04Z","timestamp":1791546184825,"version":"4.3.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2019,6,12]],"date-time":"2019-06-12T00:00:00Z","timestamp":1560297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16F16105"],"award-info":[{"award-number":["16F16105"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K18783"],"award-info":[{"award-number":["15K18783"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K08021"],"award-info":[{"award-number":["16K08021"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K19258"],"award-info":[{"award-number":["18K19258"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19H03120"],"award-info":[{"award-number":["19H03120"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2019,6,26]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">Babesia bovis<\/jats:named-content>\n            is the most virulent cause of bovine babesiosis worldwide. The disease consequences are death, abortion, and economical loss due to reduced milk and meat production. Available vaccines are not effective, treatment options are limited, and emergence of drug and acaricide resistance has been reported from different regions. There is an urgent need to identify new drug and vaccine targets. Greater than half of the genes in\n            <jats:named-content content-type=\"genus-species\">B. bovis<\/jats:named-content>\n            genome, including several expanded gene families which are unique for\n            <jats:italic>Babesia<\/jats:italic>\n            spp., have no predicted function. The available genetic engineering tools are based on conventional homologous recombination, which is time-consuming and inefficient. In this study, we adapted the CRISPR\/Cas9 system as a robust genetic engineering tool for\n            <jats:named-content content-type=\"genus-species\">B. bovis<\/jats:named-content>\n            . This advancement will aid future functional studies of uncharacterized genes.\n          <\/jats:p>","DOI":"10.1128\/msphere.00109-19","type":"journal-article","created":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T15:42:25Z","timestamp":1560267745000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Genome Editing of\n            <i>Babesia bovis<\/i>\n            Using the CRISPR\/Cas9 System"],"prefix":"10.1128","volume":"4","author":[{"given":"Hassan","family":"Hakimi","sequence":"first","affiliation":[{"name":"Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Ishizaki","sequence":"additional","affiliation":[{"name":"Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan"},{"name":"Program for Nurturing 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