{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T23:43:00Z","timestamp":1792885380906,"version":"4.5.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T00:00:00Z","timestamp":1589328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["5OD11083-7"],"award-info":[{"award-number":["5OD11083-7"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["1K01TW009981"],"award-info":[{"award-number":["1K01TW009981"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["5R21AI107135"],"award-info":[{"award-number":["5R21AI107135"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["RHD084339"],"award-info":[{"award-number":["RHD084339"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,6,24]]},"abstract":"<jats:p>\n            Brucellosis is one of the most commonly reported zoonotic disease with a worldwide distribution. Of the 12\n            <jats:italic>Brucella<\/jats:italic>\n            species,\n            <jats:named-content content-type=\"genus-species\">Brucella melitensis<\/jats:named-content>\n            is considered the most virulent and causes reproductive failure (abortions\/stillbirths) in small ruminants, which can spread the disease to other animals or to humans. Vaccination of small ruminants is a key measure used to protect both human and animal health. However, the commercially available live-attenuated vaccine for\n            <jats:named-content content-type=\"genus-species\">Brucella melitensis<\/jats:named-content>\n            Rev. 1 retains virulence and can cause disease in animals and humans. In order to evaluate the safety and efficacy in sheep, we vaccinated pregnant sheep with 16M\n            <jats:italic>\u0394vjbR<\/jats:italic>\n            . Our results indicate that 16M\n            <jats:italic>\u0394vjbR<\/jats:italic>\n            was safer for use during pregnancy, provided a similar level of protection as Rev. 1, and could be considered an improved candidate for future vaccine trials.\n          <\/jats:p>","DOI":"10.1128\/msphere.00120-20","type":"journal-article","created":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T14:42:13Z","timestamp":1589294533000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Vaccine Candidate Brucella melitensis 16M\n            <i>\u0394vjbR<\/i>\n            Is Safe in a Pregnant Sheep Model and Confers Protection"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8520-0279","authenticated-orcid":false,"given":"Martha E.","family":"Hensel","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine and Biomedical Sciences, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Garcia-Gonzalez","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Biomedical Sciences, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sankar P.","family":"Chaki","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Biomedical Sciences, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Airn","family":"Hartwig","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul W.","family":"Gordy","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Bowen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Ficht","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Biomedical Sciences, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Arenas-Gamboa","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Biomedical Sciences, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.02049"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2010.10.003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-1135(02)00255-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.08.058"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(67)90038-2"},{"key":"e_1_3_2_8_2","first-page":"177","article-title":"Conjunctival vaccination of pregnant ewes and goats with Brucella melitensis Rev 1 vaccine: safety and serological responses","volume":"23","author":"Zundel E","year":"1992","unstructured":"Zundel E, Verger JM, Grayon M, Michel R. 1992. Conjunctival vaccination of pregnant ewes and goats with Brucella melitensis Rev 1 vaccine: safety and serological responses. Ann Rech Vet 23:177\u2013188.","journal-title":"Ann Rech Vet"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0167-5877(96)01110-5"},{"key":"e_1_3_2_10_2","first-page":"409","article-title":"Immunization with viable Brucella organisms. Results of a safety test in humans","volume":"26","author":"Spink WW","year":"1962","unstructured":"Spink WW, Hall JW, III, Finstad J, Mallet E. 1962. Immunization with viable Brucella organisms. Results of a safety test in humans. 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