{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:22:15Z","timestamp":1792678935198,"version":"4.5.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,6,13]],"date-time":"2018-06-13T00:00:00Z","timestamp":1528848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,6,27]],"date-time":"2018-06-27T00:00:00Z","timestamp":1530057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI125841"],"award-info":[{"award-number":["R01AI125841"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005624","name":"PATH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005624","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2018,6,27]]},"abstract":"<jats:p>\n            <jats:italic>Shigella<\/jats:italic>\n            is an important cause of diarrhea worldwide, and efforts are ongoing to produce a safe and effective\n            <jats:italic>Shigella<\/jats:italic>\n            vaccine. Although a clear immune correlate of protection has not been established, antibodies with bactericidal capacity may provide one means of protecting against shigellosis. Thus, it is important to measure the functional capacity of antibodies, as opposed to only binding activity. This article describes a simple, robust, and high-throughput serum bactericidal assay capable of measuring\n            <jats:italic>Shigella<\/jats:italic>\n            -specific functional antibodies\n            <jats:italic>in vitro<\/jats:italic>\n            . We show for the first time that this assay was successfully performed by multiple laboratories and generated highly comparable results, particularly when SBA titers were normalized using a reference standard. The serum bactericidal assay, along with a reference serum, should greatly facilitate\n            <jats:italic>Shigella<\/jats:italic>\n            vaccine development.\n          <\/jats:p>","DOI":"10.1128\/msphere.00146-18","type":"journal-article","created":{"date-parts":[[2018,6,12]],"date-time":"2018-06-12T10:01:46Z","timestamp":1528797706000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Development, Interlaboratory Evaluations, and Application of a Simple, High-Throughput\n            <i>Shigella<\/i>\n            Serum Bactericidal Assay"],"prefix":"10.1128","volume":"3","author":[{"given":"Moon H.","family":"Nahm","sequence":"first","affiliation":[{"name":"University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jigui","family":"Yu","sequence":"additional","affiliation":[{"name":"University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailey P.","family":"Weerts","sequence":"additional","affiliation":[{"name":"Department of Enteric Infections, Bacterial Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Wenzel","sequence":"additional","affiliation":[{"name":"PATH, Washington, DC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chitradevi S.","family":"Tamilselvi","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development, University of Maryland, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakshmi","family":"Chandrasekaran","sequence":"additional","affiliation":[{"name":"Department of Enteric Infections, Bacterial Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, 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Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure, p 83\u201391. In Whistler RL, Wolfan ML (ed), Methods in carbohydrate chemistry. 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