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Here, to explore additional functions of\n              <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n              Rap1, we performed RNA sequencing analysis. Experimental validations further showed that Rap1 plays a role in iron regulation, especially under low-iron conditions. Moreover, Rap1 was involved in iron acquisition and modulation of iron-related genes. Rap1 was found to be associated with fluconazole resistance in a low-iron condition. Finally, we demonstrated that the deletion of\n              <jats:italic toggle=\"yes\">RAP1<\/jats:italic>\n              leads to reduced\n              <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n              virulence in a mouse model of infection. Together, this study reveals new functions of\n              <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n              Rap1, particularly in iron homeostasis, azole resistance, and virulence.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n              is an important pathogenic fungus that can cause superficial to life-threatening infections. Iron is essential for almost all organisms, yet it is highly restricted within the human host to defend against pathogens. To grow and survive in the iron-limited host environment,\n              <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n              has evolved multiple iron acquisition mechanisms. Understanding the regulation of iron homeostasis is, therefore, critical for elucidating\n              <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n              pathogenesis and virulence. This study explores the novel functions of\n              <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n              Rap1, with a focus on its contribution to iron acquisition and utilization. Our findings further highlight how iron availability impacts antifungal resistance and virulence through Rap1, providing insight into the complex iron regulatory machinery of\n              <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00155-25","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T13:01:33Z","timestamp":1745413293000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Deletion of\n            <i>RAP1<\/i>\n            affects iron homeostasis, azole resistance, and virulence in\n            <i>Candida albicans<\/i>"],"prefix":"10.1128","volume":"10","author":[{"given":"Min-Chi","family":"Yang","sequence":"first","affiliation":[{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University","place":["Hsinchu, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Luen","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University","place":["Hsinchu, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsuan-Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University","place":["Hsinchu, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-Huey","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, National Tsing Hua University","place":["Hsinchu, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Shan","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, National Chung Hsing University","place":["Taichung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo-Yun","family":"Tseng","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University","place":["Hsinchu, Taiwan"]},{"name":"Taiwan Mycology Reference Center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes","place":["Miaoli County, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8864-5214","authenticated-orcid":false,"given":"Hsiu-Jung","family":"Lo","sequence":"additional","affiliation":[{"name":"Taiwan Mycology Reference Center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes","place":["Miaoli County, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4398-2975","authenticated-orcid":false,"given":"I-Ching","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, National Tsing Hua University","place":["Hsinchu, Taiwan"]},{"name":"Department of Life Science, National Tsing Hua University","place":["Hsinchu, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6873-6570","authenticated-orcid":false,"given":"Chi-Jan","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, National Chung Hsing University","place":["Taichung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1533-3049","authenticated-orcid":true,"given":"Chung-Yu","family":"Lan","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, National Tsing Hua University","place":["Hsinchu, Taiwan"]},{"name":"Department of Life Science, National Tsing Hua University","place":["Hsinchu, Taiwan"]},{"name":"School of Medicine, National Tsing Hua University","place":["Hsinchu, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.07.002"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.2019950"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00960-9"},{"key":"e_1_3_4_5_2","unstructured":"WHO. 2022. 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