{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:33:52Z","timestamp":1787016832531,"version":"build-2736575974"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T00:00:00Z","timestamp":1559692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"St. Louis College of Pharmacy, Faculty Researcy Incentive Fund","award":["106"],"award-info":[{"award-number":["106"]}]},{"name":"St. Louis College of Pharmacy, Faculty Researcy Incentive Fund","award":["165"],"award-info":[{"award-number":["165"]}]},{"name":"St. Louis College of Pharmacy, Faculty Research Incentive Fund","award":["171"],"award-info":[{"award-number":["171"]}]},{"name":"Pennsylvania Academy of Sciences"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2019,6,26]]},"abstract":"<jats:p>\n                    Currently, there is very little known about the phenotypic variability within species of\n                    <jats:italic>Rhodotorula<\/jats:italic>\n                    strains and the role of their capsule.\n                    <jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>\n                    has been considered the only encapsulated human fungal pathogen, but as more individuals come to live in states of immunocompromised health, they are more susceptible to fungal infections, including those by\n                    <jats:italic>Rhodotorula<\/jats:italic>\n                    .\n                    <jats:named-content content-type=\"genus-species\">R. mucilaginosa<\/jats:named-content>\n                    species are some of those most commonly associated with clinical infections. We wanted to know if clinical and environmental strains of\n                    <jats:named-content content-type=\"genus-species\">R. mucilaginosa<\/jats:named-content>\n                    demonstrated disparate capsule phenotypes. With limited antifungal options available and clinical\n                    <jats:italic>Rhodotorula<\/jats:italic>\n                    spp. often resistant to common antifungal drugs such as fluconazole, caspofungin (1, 2), and voriconazole (2), a better understanding of the fungal biology could inform the design and use of future antifungal drugs. The generation of an antibody specific to\n                    <jats:italic>Rhodotorula<\/jats:italic>\n                    fungi could be a useful diagnostic tool, and this work presents the first mention of such in the literature.\n                  <\/jats:p>","DOI":"10.1128\/msphere.00166-19","type":"journal-article","created":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T11:42:25Z","timestamp":1560253345000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Cell Envelope Integrity and Capsule Characterization of Rhodotorula mucilaginosa Strains from Clinical and Environmental Sources"],"prefix":"10.1128","volume":"4","author":[{"given":"Johnathan","family":"Yockey","sequence":"first","affiliation":[{"name":"St. Louis College of Pharmacy, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Andres","sequence":"additional","affiliation":[{"name":"St. Louis College of Pharmacy, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moleigh","family":"Carson","sequence":"additional","affiliation":[{"name":"St. Louis College of Pharmacy, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5867-007X","authenticated-orcid":false,"given":"Jeramia J.","family":"Ory","sequence":"additional","affiliation":[{"name":"St. Louis College of Pharmacy, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy J.","family":"Reese","sequence":"additional","affiliation":[{"name":"St. Louis College of Pharmacy, St. Louis, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01747-08"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4103\/0972-2327.44561"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mmcr.2014.08.006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/465717"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1130-1406(08)70032-9"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.5144\/0256-4947.1999.533"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.41.11.5233-5235.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.476-478.2005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki020"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01647-12"},{"key":"e_1_3_2_12_2","volume-title":"Medically important fungi: a guide to identification","author":"Larone DH","year":"2002","unstructured":"Larone DH. 2002. Medically important fungi: a guide to identification, 4th ed. ASM Press, Washington, DC.","edition":"4"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1139\/w99-022"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.5.2179-2183.1999"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.2.465-471.1999"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/ec.2.4.655-663.2003"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04843.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00399-06"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004908"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.163.3.1180-1185.1985"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1177\/31.6.6841974"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00245214"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12896"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05300.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007126"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00146-08"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00290-13"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.68.2.443-448.2000"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.2.377"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.11.6155-6164.2003"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1251\/bpo68"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03780.x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05551.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707913200"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01950-13"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1117018109"},{"key":"e_1_3_2_37_2","unstructured":"Vilgalys Mycology Lab. 2019. Conserved primer sequences for PCR amplification of fungal rDNA. https:\/\/sites.duke.edu\/vilgalyslab\/rdna_primers_for_fungi\/."},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.2.4.16136"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780199634491.001.0001","volume-title":"Carbohydrate analysis: a practical approach","author":"Chaplin MF","year":"1994","unstructured":"Chaplin MF, Kennedy JF (ed). 1994. Carbohydrate analysis: a practical approach. IRL Press, New York, NY."},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20033776"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00166-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00166-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T11:17:17Z","timestamp":1721387837000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00166-19"}},"subtitle":[],"editor":[{"given":"Aaron P.","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,6,26]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,6,26]]}},"alternative-id":["10.1128\/mSphere.00166-19"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00166-19","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,26]]},"assertion":[{"value":"2019-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00166-19"}}