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Cases in dogs in the United States have been increasing due to the persistent nature of the bacterium, deficiencies in current diagnostic testing, and, most importantly, the lack of a protective vaccine. Current estimates place the seroprevalence of<jats:named-content content-type=\"genus-species\">B. canis<\/jats:named-content>in the southern United States at 7% to 8%, but with the unprecedented rates of animals moving across state and international borders and the lack of federal regulations in regard to testing, the true seroprevalence of<jats:named-content content-type=\"genus-species\">B. canis<\/jats:named-content>in the United States may very well be higher. Vaccination represents the most effective method of brucellosis control and, in response to the demand for a vaccine against<jats:named-content content-type=\"genus-species\">B. canis<\/jats:named-content>, we have developed the live attenuated<jats:named-content content-type=\"genus-species\">B. canis<\/jats:named-content>RM6\/66 \u0394<jats:italic>vjbR<\/jats:italic>vaccine strain capable of protecting mice against challenge.<\/jats:p>","DOI":"10.1128\/msphere.00172-20","type":"journal-article","created":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T13:20:06Z","timestamp":1589894406000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of the Efficacy of the Brucella canis RM6\/66 \u0394<i>vjbR<\/i>Vaccine Candidate for Protection against B. canis Infection in Mice"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0746-5949","authenticated-orcid":false,"given":"Lauren W.","family":"Stranahan","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sankar P.","family":"Chaki","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Garcia-Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar H.","family":"Khalaf","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology &amp; Poultry Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Arenas-Gamboa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&amp;M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Brucellosis in humans and animals: WHO guidance","author":"Corbel MJ","year":"2006","unstructured":"Corbel MJ. 2006. 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