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The common human parasite<jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>replicates exclusively in a vacuole in a host cell and alters its host cell\u2019s environment through secreted proteins. One of these secreted proteins, MAF1b, acts to concentrate mitochondria around the parasite\u2019s vacuole, and this relocalization alters the host immune response. Many other intracellular pathogens also recruit host mitochondria, but the identities of the partners that mediate this interaction have not previously been described in any infection. Here, we show that<jats:italic>Toxoplasma<\/jats:italic>MAF1b binds to the multifunctional MIB protein complex on the host mitochondria. Reducing the levels of the proteins in this mitochondrial complex reduces the close association of host cell mitochondria and the parasite\u2019s vacuole. This work provides new insight into a key host-pathogen interaction and identifies possible targets for future therapeutic intervention as well as a more molecular understanding of important biology.<\/jats:p>","DOI":"10.1128\/msphere.00183-17","type":"journal-article","created":{"date-parts":[[2017,5,25]],"date-time":"2017-05-25T00:35:17Z","timestamp":1495672517000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Toxoplasma gondii MAF1b Binds the Host Cell MIB Complex To Mediate Mitochondrial Association"],"prefix":"10.1128","volume":"2","author":[{"given":"Felice D.","family":"Kelly","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian M.","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja M.","family":"Cygan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle L.","family":"Parker","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin J.","family":"Boulanger","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Boothroyd","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.06.002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004644"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2858"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05395"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1126867"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.9.5687"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.18.4926"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.9.2131"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4591832.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.6.2108"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00313-12"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2015.1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.02.002"},{"key":"e_1_3_2_15_2","first-page":"356","article-title":"Morphological studies of the association of mitochondria with chlamydial inclusions and the fusion of chlamydial inclusions","volume":"40","author":"Matsumoto A","year":"1991","unstructured":"Matsumoto A Bessho H Uehira K Suda T . 1991. 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