{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:56:26Z","timestamp":1790423786336,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Food Research Institute","award":["233PRJ75PW"],"award-info":[{"award-number":["233PRJ75PW"]}]},{"name":"UW-Madison Graduate School","award":["135AAA2218"],"award-info":[{"award-number":["135AAA2218"]}]},{"name":"UW-Madison ICTR funded by Natl. Center for Advancing Translational Science","award":["UL1TR000427"],"award-info":[{"award-number":["UL1TR000427"]}]},{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["2018-6717-27523"],"award-info":[{"award-number":["2018-6717-27523"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,6,24]]},"abstract":"<jats:p>In humans, nonalcoholic fatty liver disease (NAFLD) is the most prevalent liver disease due to the increased prevalence of obesity. While treatment of NAFLD is often geared toward lifestyle changes, such as diet and exercise, the use of dietary supplements such as probiotics is underinvestigated. Here, we report that probiotic<jats:named-content content-type=\"genus-species\">Lactobacillus reuteri<\/jats:named-content>reduces fatty liver in a mouse model of diet-induced obesity. This phenotype was further enhanced upon delivery of recombinant interleukin-22 by engineered<jats:named-content content-type=\"genus-species\">Lactobacillus reuteri<\/jats:named-content>. These observations pave the road to a better understanding of probiotic mechanisms driving the reduction of diet-induced steatosis and to development of next-generation probiotics for use in the clinic. Ultimately, these studies may lead to rational selection of (engineered) probiotics to ameliorate fatty liver disease.<\/jats:p>","DOI":"10.1128\/msphere.00183-20","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T13:42:13Z","timestamp":1593006133000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Secretion of Recombinant Interleukin-22 by Engineered Lactobacillus reuteri Reduces Fatty Liver Disease in a Mouse Model of Diet-Induced Obesity"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9033-8539","authenticated-orcid":false,"given":"Jee-Hwan","family":"Oh","sequence":"first","affiliation":[{"name":"Department of Food Science, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn L.","family":"Schueler","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donnie S.","family":"Stapleton","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura M.","family":"Alexander","sequence":"additional","affiliation":[{"name":"Department of Food Science, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Liang Eric","family":"Yen","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark P.","family":"Keller","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan D.","family":"Attie","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4649-5726","authenticated-orcid":false,"given":"Jan-Peter","family":"van Pijkeren","sequence":"additional","affiliation":[{"name":"Department of Food Science, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000111245.75752.C6"},{"key":"e_1_3_2_3_2","first-page":"1039","article-title":"AHA and NHLBI review diagnosis and management of the metabolic syndrome","volume":"74","author":"Armstrong C","year":"2006","unstructured":"Armstrong C. 2006. AHA and NHLBI review diagnosis and management of the metabolic syndrome. Am Fam Physician 74:1039\u20131047.","journal-title":"Am Fam Physician"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01155"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13564"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-014-0727-3"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4103\/0976-500X.130042"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2014.06.004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.4049\/jimmunol.148.4.1176","article-title":"Expression of human IL-1 beta in Salmonella typhimurium. A model system for the delivery of recombinant therapeutic proteins in vivo","volume":"148","author":"Carrier M","year":"1992","unstructured":"Carrier M, Chatfield S, Dougan G, Nowicka U, O\u2019Callaghan D, Beesley J, Milano S, Cillari E, Liew F. 1992. Expression of human IL-1 beta in Salmonella typhimurium. A model system for the delivery of recombinant therapeutic proteins in vivo. J Immunol 148:1176\u20131181.","journal-title":"J Immunol"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.7.3183-3189.1998"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2006.03.028"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2018-317232"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku623"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00607-17"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02335-18"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-804024-9.00008-2"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.112664"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(19)30039-1"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.11743"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2019.00139"},{"key":"e_1_3_2_21_2","first-page":"488","article-title":"Interleukin-22 drives the proliferation, migration and osteogenic differentiation of mesenchymal stem cells: a novel cytokine that could contribute to new bone formation in spondyloarthropathies","volume":"56","author":"El-Zayadi AA","year":"2016","unstructured":"El-Zayadi AA, Jones EA, Churchman SM, Baboolal TG, Cuthbert RJ, El-Jawhari JJ, Badawy AM, Alase AA, El-Sherbiny YM, McGonagle D. 2016. Interleukin-22 drives the proliferation, migration and osteogenic differentiation of mesenchymal stem cells: a novel cytokine that could contribute to new bone formation in spondyloarthropathies. Rheumatology 56:488\u2013493.","journal-title":"Rheumatology"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00235"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01579282"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031951"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/sb4001992"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.223"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00183-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00183-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T21:55:46Z","timestamp":1696283746000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00183-20"}},"subtitle":[],"editor":[{"given":"Maria L.","family":"Marco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,6,24]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,6,24]]}},"alternative-id":["10.1128\/mSphere.00183-20"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00183-20","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,24]]},"assertion":[{"value":"2020-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00183-20"}}