{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T20:13:42Z","timestamp":1767730422601,"version":"build-2238731810"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T00:00:00Z","timestamp":1479254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,12,28]],"date-time":"2016-12-28T00:00:00Z","timestamp":1482883200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["1R01 AI089920-01A1"],"award-info":[{"award-number":["1R01 AI089920-01A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2016,12,28]]},"abstract":"<jats:p>\n                    Synergy between AmB and nucleotide biosynthetic pathways has been documented, but the mechanism of this interaction has not been delineated. Results from this study suggest a correlation between uridine nucleotide biosynthesis and cell integrity likely mediated through the pool of nucleotide-sugar conjugates, which are precursor molecules for both capsule and cell wall of\n                    <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n                    . Thus, we propose a mechanism by which structural defects in the cell wall resulting from perturbation of pyrimidine biosynthesis allow faster and increased penetration of AmB molecules into the cell membrane. Overall, our work demonstrates that impairment of pyrimidine biosynthesis in\n                    <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n                    could be a potential target for antifungal therapy, either alone or in combination with AmB.\n                  <\/jats:p>","DOI":"10.1128\/msphere.00191-16","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T22:50:35Z","timestamp":1479336635000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["<i>De Novo<\/i>\n                    Pyrimidine Biosynthesis Connects Cell Integrity to Amphotericin B Susceptibility in Cryptococcus neoformans"],"prefix":"10.1128","volume":"1","author":[{"given":"Dithi","family":"Banerjee","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, The State University of New York, Buffalo, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy C.","family":"Umland","sequence":"additional","affiliation":[{"name":"Department of Structural Biology, Hauptman Woodward Medical Research Institute, University at Buffalo, The State University of New York, Buffalo, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Panepinto","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, The State University of New York, Buffalo, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e328322ffac"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/649858"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(00)00220-X"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/313757"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16301-0"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197907193010303"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.10.3361-3364.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002957"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.19.8966"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)00077-0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi971628y"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206854200"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi001810q"},{"key":"e_1_3_2_15_2","first-page":"1660","article-title":"Brequinar potentiates 5-fluorouracil antitumor activity in a murine model colon 38 tumor by tissue-specific modulation of uridine nucleotide pools","volume":"52","author":"Pizzorno G","year":"1992","unstructured":"Pizzorno G Wiegand RA Lentz SK Handschumacher RE . 1992. Brequinar potentiates 5-fluorouracil antitumor activity in a murine model colon 38 tumor by tissue-specific modulation of uridine nucleotide pools. Cancer Res52:1660\u20131665.","journal-title":"Cancer Res"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152610791163336"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2014.06.003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087246"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02569-06"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.05.017"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.120055"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-08-0664"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2006.05327.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.838-840.1999"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.62.081307.162753"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2164(09)01204-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(01)00297-8"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/41.3.397"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.02.007"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.5.1249-1260.2004"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/eos.12270"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20120097"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00100-13"},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"512","DOI":"10.4049\/jimmunol.157.2.512","article-title":"Sequential acquisition of mitochondrial and plasma membrane alterations during early lymphocyte apoptosis","volume":"157","author":"Castedo M","year":"1996","unstructured":"Castedo M Hirsch T Susin SA Zamzami N Marchetti P Macho A Kroemer G . 1996. Sequential acquisition of mitochondrial and plasma membrane alterations during early lymphocyte apoptosis. J Immunol157:512\u2013521.","journal-title":"J Immunol"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-11-89"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04843.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.11.1902-1912.2005"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00446-06"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2004.08.013"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.8.1914"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(03)00117-3"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.36.9.1799"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa6645"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.3.1101-1108.1992"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00309-09"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E04-11-0987"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018769"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23048"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.5.1405-1411.1993"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.9.4538"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku340"}],"updated-by":[{"DOI":"10.1128\/msphere.00406-23","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000}}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00191-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00191-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T19:18:22Z","timestamp":1692559102000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00191-16"}},"subtitle":[],"editor":[{"given":"J. Andrew","family":"Alspaugh","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,12,28]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,12,28]]}},"alternative-id":["10.1128\/mSphere.00191-16"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00191-16","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,28]]},"assertion":[{"value":"2016-07-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-10-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00191-16"}}