{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:42:10Z","timestamp":1792114930764,"version":"4.4.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2017,6,14]],"date-time":"2017-06-14T00:00:00Z","timestamp":1497398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T00:00:00Z","timestamp":1498608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000052","name":"HHS | NIH | NIH Office of the Director","doi-asserted-by":"publisher","award":["1R15 AI119801-01"],"award-info":[{"award-number":["1R15 AI119801-01"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2017,6,28]]},"abstract":"<jats:p>\n                    Azoles are antifungals that are widely utilized due to relatively low toxicity and cost of treatment. One of their drawbacks, however, is that azoles are primarily cytostatic, leaving fungal cells capable of developing drug resistance. The human pathogen\n                    <jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>\n                    acquires resistance to the azole drug fluconazole (FLC) through the development of aneuploidy, leading to elevated expression of key resistance genes, a mechanism that is also common for\n                    <jats:named-content content-type=\"genus-species\">Candida albicans<\/jats:named-content>\n                    (K. J. Kwon-Chung and Y. C. Chang, PLoS Pathog 8:e1003022, 2012,\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1371\/journal.ppat.1003022\">https:\/\/doi.org\/10.1371\/journal.ppat.1003022<\/jats:ext-link>\n                    ; J. Morschh\u00e4user, J Microbiol 54:192\u2013201, 2016,\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1007\/s12275-016-5628-4\">https:\/\/doi.org\/10.1007\/s12275-016-5628-4<\/jats:ext-link>\n                    ). However, the exact ways in which FLC contributes to increased resistance in either of these important fungal pathogens remain unclear. Here we found that FLC treatment leads to an increase in DNA content in\n                    <jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>\n                    through multiple mechanisms, potentially increasing the size of a pool of cells from which aneuploids with increased resistance are selected. This study demonstrated the importance of FLC\u2019s inhibitory effects on growth and cytokinesis in the generation of cell populations with decreased sensitivity to the drug.\n                  <\/jats:p>","DOI":"10.1128\/msphere.00205-17","type":"journal-article","created":{"date-parts":[[2017,6,14]],"date-time":"2017-06-14T20:35:12Z","timestamp":1497472512000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Fluconazole-Induced Ploidy Change in Cryptococcus neoformans Results from the Uncoupling of Cell Growth and Nuclear Division"],"prefix":"10.1128","volume":"2","author":[{"given":"Sophie","family":"Altamirano","sequence":"first","affiliation":[{"name":"Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Genetics 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