{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T20:48:59Z","timestamp":1790455739588,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2016,8,24]],"date-time":"2016-08-24T00:00:00Z","timestamp":1471996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,8,31]],"date-time":"2016-08-31T00:00:00Z","timestamp":1472601600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["F32 AI100501"],"award-info":[{"award-number":["F32 AI100501"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K22 AI110552"],"award-info":[{"award-number":["K22 AI110552"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004917","name":"Cancer Prevention and Research Institute of Texas","doi-asserted-by":"publisher","award":["RR150044"],"award-info":[{"award-number":["RR150044"]}],"id":[{"id":"10.13039\/100004917","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2016,8,31]]},"abstract":"<jats:p>\n            Despite intense research effort from scientists and the advent of the molecular age of biomedical research, many of the mechanisms that underlie pathogenesis are still understood poorly, if at all. The opportunistic human pathogen\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>\n            causes a variety of soft tissue infections and is responsible for over 50,000 hospital-acquired infections per year. In addition,\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            exhibits a striking degree of innate and acquired antimicrobial resistance, complicating treatment. It is increasingly important to understand\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            virulence. In an effort to gain this information in an unbiased fashion, we used a high-throughput phenotypic screen to identify small molecules that disrupted bacterial pathogenesis and increased host survival using the model nematode\n            <jats:named-content content-type=\"genus-species\">Caenorhabditis elegans<\/jats:named-content>\n            . This method led to the unexpected discovery that addition of a modified nucleotide, 5-fluorouridine, disrupted bacterial RNA metabolism and inhibited synthesis of pyoverdine, a critical toxin. Our results demonstrate that this compound specifically functions as an antivirulent.\n          <\/jats:p>","DOI":"10.1128\/msphere.00217-16","type":"journal-article","created":{"date-parts":[[2016,8,25]],"date-time":"2016-08-25T01:59:29Z","timestamp":1472090369000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["A High-Content, Phenotypic Screen Identifies Fluorouridine as an Inhibitor of Pyoverdine Biosynthesis and Pseudomonas aeruginosa Virulence"],"prefix":"10.1128","volume":"1","author":[{"given":"Daniel R.","family":"Kirienko","sequence":"first","affiliation":[{"name":"Department of BioSciences, Rice University, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey V.","family":"Revtovich","sequence":"additional","affiliation":[{"name":"Department of BioSciences, Rice University, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1537-4967","authenticated-orcid":false,"given":"Natalia V.","family":"Kirienko","sequence":"additional","affiliation":[{"name":"Department of BioSciences, Rice University, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01305.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2014.09.008"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2014.914172"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.08.002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1465-0691.2004.1006.x"},{"key":"e_1_3_2_7_2","first-page":"651","article-title":"A retrospective on the failures and successes of antibacterial drug discovery","volume":"8","author":"Silver LL","year":"2005","unstructured":"Silver LL . 2005. 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