{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:14:17Z","timestamp":1783523657913,"version":"3.55.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:00:00Z","timestamp":1692835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:00:00Z","timestamp":1692835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"UB | Institute of Veterinary Bacteriology, University of Bern","award":["REF-660-50"],"award-info":[{"award-number":["REF-660-50"]}]},{"name":"Clinical Research Platform, Vetsuisse Faculty, University of Bern","award":["REF-600-19"],"award-info":[{"award-number":["REF-600-19"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2023,8,24]]},"abstract":"ABSTRACT<\/jats:title>\n \n Whole-genome sequence analysis of a macrolide, lincosamide, streptogramin B (MLS\n B<\/jats:sub>\n )-resistant\n Trueperella pyogenes<\/jats:italic>\n from a dog revealed a new 23S ribosomal RNA methylase gene\n erm<\/jats:italic>\n (56). Expression of the cloned\n erm<\/jats:italic>\n (56) confers resistance to MLS\n B<\/jats:sub>\n in\n T. pyogenes<\/jats:italic>\n and\n Escherichia coli<\/jats:italic>\n . The\n erm<\/jats:italic>\n (56) gene was flanked by two IS\n 6100<\/jats:italic>\n integrated on the chromosome next to a\n sul1<\/jats:italic>\n -containing class 1 integron. GenBank query revealed additional\n erm<\/jats:italic>\n (56)-containing elements in another\n T. pyogenes<\/jats:italic>\n and in\n Rothia nasimurium<\/jats:italic>\n from livestock.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n A novel 23S ribosomal RNA methylase gene\n erm<\/jats:italic>\n (56) flanked by insertion sequence IS\n 6100<\/jats:italic>\n was identified in a\n Trueperella pyogenes<\/jats:italic>\n isolated from the abscess of a dog and was also present in another\n T. pyogenes<\/jats:italic>\n and in\n Rothia nasimurium<\/jats:italic>\n from livestock. It was shown to confer resistance to macrolide, lincosamide, streptogramin B antibiotics in\n T. pyogenes<\/jats:italic>\n and\n E. coli,<\/jats:italic>\n indicating functionality in both Gram-positive and Gram-negative bacteria. The detection of\n erm<\/jats:italic>\n (56) on different elements in unrelated bacteria from different animal sources and geographical origins suggests that it has been independently acquired and likely selected by the use of antibiotics in animals.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/msphere.00239-23","type":"journal-article","created":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T13:01:38Z","timestamp":1688734898000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Novel macrolide-lincosamide-streptogramin B resistance gene\n erm<\/i>\n (56) in\n Trueperella pyogenes<\/i>"],"prefix":"10.1128","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9113-5829","authenticated-orcid":true,"given":"Emma","family":"Marchionatti","sequence":"first","affiliation":[{"name":"Division of Molecular Bacterial Epidemiology and Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern , Bern, Switzerland"},{"name":"Clinic for Ruminants, Department of Clinical Veterinary Science, Vetsuisse Faculty, University of Bern , Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5722-9445","authenticated-orcid":true,"given":"Vincent","family":"Perreten","sequence":"additional","affiliation":[{"name":"Division of Molecular Bacterial Epidemiology and Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern , Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20112737"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ARBA-0021-2017"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01145.x"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/324626"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2020.108602"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2019.12.008"},{"key":"e_1_3_4_8_2","volume-title":"CLSI guideline M45","year":"2016","unstructured":"Clinical and Laboratory Standards Institute (CLSI). 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