{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T17:03:33Z","timestamp":1788714213343,"version":"build-2803163510"},"reference-count":72,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI19641"],"award-info":[{"award-number":["R01AI19641"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Clinical and Translational Science Award, University at Buffalo","award":["UL1TR001412"],"award-info":[{"award-number":["UL1TR001412"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,7,29]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Chronic obstructive pulmonary disease (COPD) is a progressive inflammatory lung disease and the third leading cause of death globally. Nontypeable\n                      <jats:italic toggle=\"yes\">Haemophilus influenzae<\/jats:italic>\n                      (NTHi) and\n                      <jats:italic toggle=\"yes\">Moraxella catarrhalis<\/jats:italic>\n                      (Mcat) are common pathogens in individuals with COPD. Acquisition of NTHi or Mcat can cause acute exacerbations of COPD. NTHi and\/or Mcat also persist for months in the lower airways and lead to chronic inflammation. We hypothesized that infection by NTHi or Mcat, singly or during coinfection, requires regulation of specific bacterial and host cell pathways. We investigated this phenomenon using an\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      cell culture model consisting of lung carcinoma H292 cell lines, infected with NTHi, Mcat, or both species. Samples were fractionated into \u201capical fluid,\u201d containing free-floating bacteria, and adhered\/invaded bacteria on or within H292 cells. We used transcriptomic profiling with RNA-seq and various bioinformatic analyses to disentangle host-pathogen interactions in epithelial cell infection from the perspective of each species. Several biological pathways were differentially regulated across all conditions (31, NTHi; 22, Mcat; and 169, human). NTHi transcriptomic profiles differed during mono-infection and coinfection; examples included downregulation of iron-sulfur metabolism (IscR regulon) and differential regulation of quorum sensing in coinfection compared to mono-infection. Mcat was comparatively less affected by the presence of NTHi during coinfection. H292 epithelial cells responded broadly to all infections with distinct responses to mono-infection and coinfection. Enriched host pathways included influenza\/interferon\/Wnt and proinflammatory responses. These findings suggest common and distinct processes involved in NTHi and\/or Mcat-induced COPD pathogenesis and have implications for therapeutic intervention.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Chronic obstructive pulmonary disease (COPD) is a leading cause of death worldwide. Bacteria such as nontypeable\n                        <jats:italic toggle=\"yes\">Haemophilus influenzae<\/jats:italic>\n                        (NTHi) and\n                        <jats:italic toggle=\"yes\">Moraxella catarrhalis<\/jats:italic>\n                        (Mcat) can cause exacerbations of COPD, and they can persist in the lungs for months, which increases inflammation. We studied how these bacteria interact with lung cells by infecting a cell culture model with NTHi, Mcat, or both. We used RNA sequencing and bioinformatic analysis to examine how the bacteria and host cells respond. When NTHi and Mcat were present together, they behaved differently than when each was alone. We found that different host biological pathways were activated during infection, including those related to inflammation and immune responses. These results provide insights into how NTHi and Mcat contribute to COPD progression and suggest potential targets for new treatments.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00242-25","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T09:00:52Z","timestamp":1749546052000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Host-pathogen interaction profiling of nontypeable\n                    <i>Haemophilus influenzae<\/i>\n                    and\n                    <i>Moraxella catarrhalis<\/i>\n                    coinfection of bronchial epithelial cells"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6995-3617","authenticated-orcid":true,"given":"Adonis","family":"D\u2019Mello","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/055yg0521","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Institute for Genome Sciences, University of Maryland School of Medicine","place":["Baltimore, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3496-2641","authenticated-orcid":true,"given":"Timothy F.","family":"Murphy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01q1z8k08","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Department of Medicine, University at Buffalo, The State University of New York","place":["Buffalo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Wade","sequence":"additional","affiliation":[{"name":"Department of Epidemiology of Microbial Diseases, Yale School of Public Heath","place":["New Haven, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charmaine","family":"Kirkham","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01q1z8k08","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Department of Medicine, University at Buffalo, The State University of New York","place":["Buffalo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Yale School of Medicine","place":["New Haven, USA"]},{"name":"Bioinformatics Resource at the W.M. Keck Foundation Biotechnology Resource Laboratory, Yale School of Medicine","place":["New Haven, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0615-3257","authenticated-orcid":false,"given":"Herv\u00e9","family":"Tettelin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/055yg0521","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Institute for Genome Sciences, University of Maryland School of Medicine","place":["Baltimore, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8145-7536","authenticated-orcid":true,"given":"Melinda M.","family":"Pettigrew","sequence":"additional","affiliation":[{"name":"Division of Environmental Health Sciences, University of Minnesota School of Public Health","place":["Minneapolis, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(21)00511-7"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/resp.14041"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61382-8"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0800353"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012561"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.12.066"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00081409"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2020.1863330"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200412-1747OC"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/599375"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2016-209023"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/172.1.132"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348949110000802"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/169.6.1312"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis922"},{"key":"e_1_3_4_17_2","volume-title":"Mandell, Douglas and Bennett\u2019s principles and practice of infectious diseases","author":"Murphy TF","year":"2020","unstructured":"Murphy TF. 2020. 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