{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:45:17Z","timestamp":1753883117774,"version":"3.41.2"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,6,6]],"date-time":"2018-06-06T00:00:00Z","timestamp":1528243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,6,27]],"date-time":"2018-06-27T00:00:00Z","timestamp":1530057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Boston Childrens Hospital Technology Development Fund","award":["Technology Development Fund"],"award-info":[{"award-number":["Technology Development Fund"]}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21 AI115303"],"award-info":[{"award-number":["R21 AI115303"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21 AI115023"],"award-info":[{"award-number":["R21 AI115023"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2018,6,27]]},"abstract":"<jats:p>\n            Diarrheal disease is the most common infectious disease of children in the developing world. Our goal is to develop a diarrheal antigen presentation platform based on whole\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            cells that does not depend on protein purification. We have previously shown the feasibility of genetically fusing antigens to the\n            <jats:named-content content-type=\"genus-species\">V.\u00a0cholerae<\/jats:named-content>\n            biofilm matrix protein RbmA for presentation on the cell surface. A mucosal adjuvant could improve immunogenicity of such a vaccine at the mucosal surface. Here we engineer a live attenuated\n            <jats:named-content content-type=\"genus-species\">V.\u00a0cholerae<\/jats:named-content>\n            vaccine to constitutively synthesize mmCT, a nontoxic form of cholera toxin. When this vaccine is delivered sublingually,\n            <jats:italic>in vivo<\/jats:italic>\n            -synthesized mmCT acts as both an adjuvant and antigen. This could greatly increase the magnitude and duration of the immune response elicited by codelivered heterologous antigens.\n          <\/jats:p>","DOI":"10.1128\/msphere.00245-18","type":"journal-article","created":{"date-parts":[[2018,12,24]],"date-time":"2018-12-24T11:10:05Z","timestamp":1545649805000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Sublingual Adjuvant Delivery by a Live Attenuated Vibrio cholerae-Based Antigen Presentation Platform"],"prefix":"10.1128","volume":"3","author":[{"given":"Julie","family":"Liao","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Boston Children\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob A.","family":"Gibson","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley S.","family":"Pickering","sequence":"additional","affiliation":[{"name":"National Centre for Foreign Animal Diseases, Canadian Food Inspection Agency, Government of Canada, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9173-8662","authenticated-orcid":false,"given":"Paula I.","family":"Watnick","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Boston Children\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.07.036"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp1712099"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)31581-7"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)60959-2"},{"key":"e_1_3_2_6_2","first-page":"638","article-title":"Vaxchora: the first FDA-approved cholera vaccination in the United States","volume":"42","author":"Mosley JF","year":"2017","unstructured":"Mosley JF, II, Smith LL, Brantley P, Locke D, Como M. 2017. Vaxchora: the first FDA-approved cholera vaccination in the United States. P T 42:638\u2013640.","journal-title":"P T"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-017-2885-8"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(16)30211-X"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00752-17"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftx126"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006399"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00224-15"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(89)90228-4"},{"key":"e_1_3_2_14_2","first-page":"188","article-title":"From cholera to enterotoxigenic Escherichia coli (ETEC) vaccine development","volume":"133","author":"Svennerholm AM","year":"2011","unstructured":"Svennerholm AM. 2011. From cholera to enterotoxigenic Escherichia coli (ETEC) vaccine development. Indian J Med Res 133:188\u2013196.","journal-title":"Indian J Med Res"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00127-12"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.06670"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126849"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0511236"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1401633"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.03.002"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.5841-5847.1999"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0157015"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002348107"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2552"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/170.2.278"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00374-13"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01407-12"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00248-16"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00536-10"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00137-7"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00464-13"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.10.069"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006999"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.32253"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002373"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053999"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2853-2856.1996"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.7.2689-2696.1995"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1996.0001"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.7.2941-2949.1997"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.8.3118-3125.1997"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.06.040"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.2.6.685-688.1995"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00245-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00245-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T04:12:47Z","timestamp":1646712767000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00245-18"}},"subtitle":[],"editor":[{"given":"Drusilla L.","family":"Burns","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,6,27]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,6,27]]}},"alternative-id":["10.1128\/mSphere.00245-18"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00245-18","relation":{},"ISSN":["2379-5042"],"issn-type":[{"type":"electronic","value":"2379-5042"}],"subject":[],"published":{"date-parts":[[2018,6,27]]},"assertion":[{"value":"2018-05-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00245-18"}}