{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:12:10Z","timestamp":1792714330260,"version":"4.5.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T00:00:00Z","timestamp":1474416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,10,26]],"date-time":"2016-10-26T00:00:00Z","timestamp":1477440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103506"],"award-info":[{"award-number":["P20GM103506"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2016,10,26]]},"abstract":"<jats:p>\n            Cholera, an infectious disease of the small intestine caused by the aquatic bacterium\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            , often results in vomiting and acute watery diarrhea. If left untreated or if the response is too slow, the symptoms can quickly lead to extreme dehydration and ultimately death of the patient. Recent anecdotal evidence of cholera patients suffering from increasingly severe symptoms and of disease progression at a much higher rate than previously observed has emerged. As recent cholera outbreaks caused by increasingly virulent strains have resulted in higher mortality rates, the need to investigate the mechanism(s) allowing this observed increased virulence is apparent. The significance of our research is in identifying the mechanism for increased virulence capabilities, which will allow the development of a model that will greatly enhance our understanding of cholera disease and\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            pathogenesis, leading to broader biomedical impacts, as cholera serves as a model for other enteric diarrheal diseases.\n          <\/jats:p>","DOI":"10.1128\/msphere.00253-16","type":"journal-article","created":{"date-parts":[[2016,9,22]],"date-time":"2016-09-22T02:19:30Z","timestamp":1474510770000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Single Nucleotide Polymorphisms in Regulator-Encoding Genes Have an Additive Effect on Virulence Gene Expression in a Vibrio cholerae Clinical Isolate"],"prefix":"10.1128","volume":"1","author":[{"given":"Bailey M.","family":"Carignan","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Plymouth State University, Plymouth, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle D.","family":"Brumfield","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Plymouth State University, Plymouth, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike S.","family":"Son","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Plymouth State University, Plymouth, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01571061"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.11150\/kansenshogakuzasshi1970.71.1037"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.11.3191-3198.1995"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90481-U"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00378-11"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.9.2833"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.4.1487"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.3134"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01286-11"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00047-10"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5270.1910"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.14.4250-4256.1999"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02518.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.2.730"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.21.6974-6980.1992"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02111.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.12.5403"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.16.1.129-134.1982"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1947-1952.2001"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00799-10"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-9688-9_1"},{"key":"e_1_3_2_23_2","first-page":"29","volume-title":"Vibrio cholerae\u2014genomics and molecular biology","author":"Morales R","year":"2008","unstructured":"Morales R Delgado G Cravioto A . 2008. 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