{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:11:08Z","timestamp":1782234668335,"version":"3.54.5"},"reference-count":76,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,8,29]],"date-time":"2018-08-29T00:00:00Z","timestamp":1535500800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Molecular Diagnostics Laboratory Auburn University","award":["104"],"award-info":[{"award-number":["104"]}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["U19 AI084024"],"award-info":[{"award-number":["U19 AI084024"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI119164"],"award-info":[{"award-number":["R01 AI119164"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2018,8,29]]},"abstract":"<jats:p>For detection of anti-<jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>antibodies by serological assays, use of classical whole-organism chlamydial antigens results in high cross-reactivity. These antigens bind mainly antibodies against the major outer membrane protein (OmpA) and bind antibodies against other immunodominant non-OmpA proteins to a lesser extent, resulting in poor assay sensitivity. The specificity of<jats:italic>C.\u00a0trachomatis<\/jats:italic>serology is also compromised by the high prevalence of cross-reactive anti-<jats:named-content content-type=\"genus-species\">C.\u00a0pneumoniae<\/jats:named-content>antibodies in human populations. We previously identified 48 highly specific<jats:named-content content-type=\"genus-species\">C.\u00a0trachomatis<\/jats:named-content>B cell epitope peptide antigens of 21 immunodominant proteins. This study validated peptide antigen-based novel ELISAs that provide highly specific and sensitive detection of anti-<jats:named-content content-type=\"genus-species\">C.\u00a0trachomatis<\/jats:named-content>antibodies. Compared to four commercial ELISAs that achieved only poor sensitivities (51.5% to 64.8%), the combined signals of 5 to 11 peptides provided high sensitivity (86.5% to 91.8%) at the same 98% specificity. Thus, by using multiple peptide antigens of immunodominant proteins, we created simple ELISAs with specificity and sensitivity superior to standard<jats:italic>C. trachomatis<\/jats:italic>serodiagnosis.<\/jats:p>","DOI":"10.1128\/msphere.00253-18","type":"journal-article","created":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T10:02:42Z","timestamp":1533031362000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Comprehensive Molecular Serology of Human<i>Chlamydia trachomatis<\/i>Infections by Peptide Enzyme-Linked Immunosorbent Assays"],"prefix":"10.1128","volume":"3","author":[{"given":"K. Shamsur","family":"Rahman","sequence":"first","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toni","family":"Darville","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali N.","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine M.","family":"O'Connell","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold C.","family":"Wiesenfeld","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynaecology and Reproductive Sciences, the University of Pittsburgh School of Medicine and the Magee-Womens Research Institute Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.","family":"Hillier","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynaecology and Reproductive Sciences, the University of Pittsburgh School of Medicine and the Magee-Womens Research Institute Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2019Ashia E.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Kaltenboeck","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"846","volume-title":"Bergey\u2019s manual of systematic bacteriology","author":"Kuo CC","year":"2011","unstructured":"Kuo CC, Stephens RS, Bavoil PM, Kaltenboeck B. 2011. Genus I. Chlamydia Jones, rake and Stearns 1945, 55AL, vol 4, p 846\u2013865. In Bergey\u2019s manual of systematic bacteriology, 2nd ed. Springer, New York, NY.","edition":"2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40588-014-0005-8"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00526.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00209-8"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmq060"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1508-0"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00099"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199506000-00002"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/514223"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(70)90096-6"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.7.3.356-360.1973"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/130.4.388"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.1.93-97.1990"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(01)00299-1"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1139\/m92-194"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.52.2.99"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-2115(98)00303-0"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.104.1.208"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/315603"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s150100050054"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/37.10.1054"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1368-1377.2001"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/41.9.1017"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004352"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0152810"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-21127-0"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2018.02.017"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151497"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00746-09"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.09.037"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.6010-6013.1998"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31805d0209"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1155\/S1064744997000616"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-49-5-457"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.2.584-587.2002"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg479"},{"key":"e_1_3_3_38_2","doi-asserted-by":"crossref","first-page":"347","DOI":"10.7883\/yoken.JJID.2007.347","article-title":"Kinetic study of antibodies (IgG, IgA) to Chlamydia trachomatis: importance of IgA antibody in screening test for C.\u00a0trachomatis infection by peptide-based enzyme immunosorbent assay","volume":"60","author":"Komoda T","year":"2007","unstructured":"Komoda T. 2007. Kinetic study of antibodies (IgG, IgA) to Chlamydia trachomatis: importance of IgA antibody in screening test for C.\u00a0trachomatis infection by peptide-based enzyme immunosorbent assay. Jpn J Infect Dis 60:347\u2013351.","journal-title":"Jpn J Infect Dis"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1258\/095646202762226119"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04365.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00021-09"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rinim.2013.05.001"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000311"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00102-15"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00010-08"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001240"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.729020"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23118-7"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90078-8"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/sim.762"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00223-07"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/sim.6803"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw485"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183101"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268899003040"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.08.022"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00235"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.4082-4085.2001"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001873"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2016.05.008"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-5113"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000432"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2011.06.021"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500343"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-372-1_6"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e3182519326"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/der167"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-016-2769-7"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-9212"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2078-8"},{"key":"e_1_3_3_72_2","unstructured":"Solomon AW. 2006. World Health Organization and International Trachoma Initiative. Trachoma control: a guide for programme managers. http:\/\/apps.who.int\/iris\/bitstream\/handle\/10665\/43405\/9241546905_eng.pdf;jsessionid=E324284267101B9F7B60C38EFA558E7F?sequence=1."},{"key":"e_1_3_3_73_2","unstructured":"Eng J. 2014. ROC analysis: Web-based calculator for ROC curves. Johns Hopkins University Baltimore MD. Updated 19 3 2014. Accessed 4 January 2017. http:\/\/www.jrocfit.org."},{"key":"e_1_3_3_74_2","first-page":"203","article-title":"Measures of diagnostic accuracy: basic definitions","volume":"19","author":"\u0160imundi\u0107 AM","year":"2009","unstructured":"\u0160imundi\u0107 AM. 2009. Measures of diagnostic accuracy: basic definitions. EJIFCC 19:203\u2013211.","journal-title":"EJIFCC"},{"key":"e_1_3_3_75_2","first-page":"132","article-title":"Evaluating measures of indicators of diagnostic test performance: fundamental meanings and formulators","volume":"3","author":"Okeh UM","year":"2012","unstructured":"Okeh UM, Okoro CN. 2012. Evaluating measures of indicators of diagnostic test performance: fundamental meanings and formulators. J Biom Biostat 3:132. https:\/\/www.omicsonline.org\/evaluating-measures-of-indicators-of-diagnostic-test-performance-fundamental-meanings-and-formulars-2155-6180.1000132.php?aid=4054.","journal-title":"J Biom Biostat"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.3109\/08958378.2014.955932"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.10326"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00246-18"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00253-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00253-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T20:31:16Z","timestamp":1720470676000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00253-18"}},"subtitle":[],"editor":[{"given":"Patricia P.","family":"Wilkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,8,29]]},"references-count":76,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,8,29]]}},"alternative-id":["10.1128\/mSphere.00253-18"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00253-18","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,29]]},"assertion":[{"value":"2018-05-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00253-18"}}