{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:50:42Z","timestamp":1790808642952,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T00:00:00Z","timestamp":1564531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T00:00:00Z","timestamp":1566950400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000052","name":"HHS | NIH | NIH Office of the Director","doi-asserted-by":"publisher","award":["R01DC003915"],"award-info":[{"award-number":["R01DC003915"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000052","name":"HHS | NIH | NIH Office of the Director","doi-asserted-by":"publisher","award":["R01GM103612"],"award-info":[{"award-number":["R01GM103612"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2019,8,28]]},"abstract":"<jats:p>\n            Multiple respiratory illnesses are associated with formation of biofilms within the human airway by NTHI. However, a substantial amount of our understanding of the mechanisms that underlie NTHI biofilm formation is obtained from\n            <jats:italic>in vitro<\/jats:italic>\n            studies. Our\n            <jats:italic>in silico<\/jats:italic>\n            model that describes biofilm formation by NTHI within the middle ears of\n            <jats:named-content content-type=\"genus-species\">Chinchilla lanigera<\/jats:named-content>\n            will help isolate processes potentially responsible for the differences between the morphologies of biofilms formed\n            <jats:italic>in vivo<\/jats:italic>\n            versus those formed\n            <jats:italic>in vitro<\/jats:italic>\n            . Thus, the\n            <jats:italic>in silico<\/jats:italic>\n            model can be used to glean mechanisms that underlie biofilm formation\n            <jats:italic>in vivo<\/jats:italic>\n            and connect those mechanisms to those obtained from\n            <jats:italic>in vitro<\/jats:italic>\n            experiments. The\n            <jats:italic>in silico<\/jats:italic>\n            model developed here can be extended to investigate potential roles of specific host responses (e.g., mucociliary clearance) on NTHI biofilm formation\n            <jats:italic>in vivo<\/jats:italic>\n            . The developed computational tools can also be used to analyze and describe biofilm formation by other bacterial species\n            <jats:italic>in vivo<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/msphere.00254-19","type":"journal-article","created":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T12:59:23Z","timestamp":1564491563000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["<i>In Silico<\/i>\n            Modeling of Biofilm Formation by Nontypeable Haemophilus influenzae\n            <i>In Vivo<\/i>"],"prefix":"10.1128","volume":"4","author":[{"given":"Jonathan R.","family":"Brown","sequence":"first","affiliation":[{"name":"Battelle Center for Mathematical Medicine, The Research Institute at the Nationwide Children\u2019s Hospital, Columbus, Ohio, 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