{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:50:16Z","timestamp":1753883416675,"version":"3.41.2"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | National Institutes of Health","award":["AI106878"],"award-info":[{"award-number":["AI106878"]}]},{"name":"HHS | National Institutes of Health","award":["AI099243"],"award-info":[{"award-number":["AI099243"]}]},{"name":"HHS | National Institutes of Health","award":["AI137164"],"award-info":[{"award-number":["AI137164"]}]},{"name":"HHS | NIH | Fogarty International Center","award":["TW005572"],"award-info":[{"award-number":["TW005572"]}]},{"name":"HHS | NIH | Fogarty International Center","award":["TW010362"],"award-info":[{"award-number":["TW010362"]}]},{"name":"Intramural Research Program of the NIH and NIDDK"},{"name":"EC | European Regional Development Fund","award":["ITMS: 313021Y920"],"award-info":[{"award-number":["ITMS: 313021Y920"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2023,10,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cholera caused by\n            <jats:italic>Vibrio cholerae<\/jats:italic>\n            O139 emerged in the early 1990s and spread rapidly to 11 Asian countries before receding for unclear reasons. Protection against cholera is serogroup-specific, which is defined by the O-specific polysaccharide (OSP) component of lipopolysaccharide (LPS).\n            <jats:italic>V. cholerae<\/jats:italic>\n            O139 also expresses the OSP-capsule. We, therefore, assessed antibody responses targeting\n            <jats:italic>V. cholerae<\/jats:italic>\n            O139 OSP, LPS, capsule, and vibriocidal responses in patients in Bangladesh with cholera caused by\n            <jats:italic>V. cholerae<\/jats:italic>\n            O139. We compared these responses to those of age-gender-blood group-matched recipients of the bivalent oral cholera vaccine (OCV O1\/O139). We found prominent OSP, LPS, and vibriocidal responses in patients, with a high correlation between these responses. OSP responses primarily targeted the terminal tetrasaccharide of OSP. Vaccinees developed OSP, LPS, and vibriocidal antibody responses, but of significantly lower magnitude and responder frequency (RF) than matched patients. We separately analyzed responses in pediatric vaccinees born after\n            <jats:italic>V. cholerae<\/jats:italic>\n            O139 had receded in Bangladesh. We found that OSP responses were boosted in children who had previously received a single dose of bivalent OCV 3 yr previously but not in vaccinated immunologically na\u00efve children. Our results suggest that OSP-specific responses occur during cholera caused by\n            <jats:italic>V. cholerae<\/jats:italic>\n            O139 despite the presence of capsules, that vaccination with bivalent OCV is poorly immunogenic in the short term in immunologically na\u00efve individuals, but that OSP-specific immune responses can be primed by previous exposure, although whether such responses can protect against O139 cholera is uncertain.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Cholera is a severe dehydrating illness in humans caused by\n              <jats:italic>Vibrio cholerae<\/jats:italic>\n              serogroups O1 or O139. Protection against cholera is serogroup-specific, which is defined by the O-specific polysaccharide (OSP) of\n              <jats:italic>V. cholerae<\/jats:italic>\n              LPS. Yet, little is known about immunity to O139 OSP. In this study, we assessed immune responses targeting OSP in patients from an endemic region with cholera caused by\n              <jats:italic>V. cholerae<\/jats:italic>\n              O139. We compared these responses to those of the age-gender-blood group-matched recipients of the bivalent oral cholera vaccine. Our results suggest that OSP-specific responses occur during cholera caused by\n              <jats:italic>V. cholerae<\/jats:italic>\n              O139 and that the OSP responses primarily target the terminal tetrasaccharide of OSP. Our results further suggest that vaccination with the bivalent vaccine is poorly immunogenic in the short term for inducing O139-specific OSP responses in immunologically na\u00efve individuals, but OSP-specific immune responses can be primed by previous exposure or vaccination.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00255-23","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T13:03:23Z","timestamp":1693400603000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of O-specific polysaccharide responses in patients following infection with\n            <i>Vibrio cholerae<\/i>\n            O139 versus vaccination with a bivalent (O1\/O139) oral killed cholera vaccine in Bangladesh"],"prefix":"10.1128","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6531-1817","authenticated-orcid":false,"given":"M. Hasanul","family":"Kaisar","sequence":"first","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research Bangladesh (icddr,b) , Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meagan","family":"Kelly","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital , Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7954-673X","authenticated-orcid":false,"given":"Mohammad","family":"Kamruzzaman","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research Bangladesh (icddr,b) , Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3755-4763","authenticated-orcid":false,"given":"Taufiqur R.","family":"Bhuiyan","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research Bangladesh (icddr,b) , Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahima","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research Bangladesh (icddr,b) , Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashraful Islam","family":"Khan","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research Bangladesh (icddr,b) , Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina C.","family":"LaRocque","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital , Boston, Massachusetts, USA"},{"name":"Department of Medicine, Harvard Medical School , Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen B.","family":"Calderwood","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital , Boston, Massachusetts, USA"},{"name":"Department of Medicine, Harvard Medical School , Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason B.","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Medicine, Harvard Medical School , Boston, Massachusetts, USA"},{"name":"Department of Pediatrics, Harvard Medical School , Boston, Massachusetts, USA"},{"name":"Division of Global Health, MassGeneral Hospital for Children , Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8881-1849","authenticated-orcid":false,"given":"Richelle C.","family":"Charles","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital , Boston, Massachusetts, USA"},{"name":"Department of Medicine, Harvard Medical School , Boston, Massachusetts, USA"},{"name":"Department of Immunology and Infectious Diseases, Harvard T.H. 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