{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:50:23Z","timestamp":1753883423439,"version":"3.41.2"},"reference-count":7,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:00:00Z","timestamp":1692835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:00:00Z","timestamp":1692835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2023,8,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Yifan Wang works in the field of molecular parasitology with a focus on host-pathogen interactions. In this mSphere of Influence article, he reflects on how papers entitled \u201cA genome-wide CRISPR screen in\n            <jats:italic>Toxoplasma<\/jats:italic>\n            identifies essential apicomplexan genes\u201d by S. M. Sidik, D. Huet, S. M. Ganesan, M.-H. Huynh, et al. (Cell 166:1423.e12\u20131435.e12, 2016,\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1016\/j.cell.2016.08.019\" xlink:type=\"simple\">https:\/\/doi.org\/10.1016\/j.cell.2016.08.019<\/jats:ext-link>\n            ) and \u201cMapping host-microbe transcriptional interactions by dual Perturb-seq\u201d by S. Butterworth, K. Kordova, S. Chandrasekaran, K. K. Thomas, et al. (bioRxiv,\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1101\/2023.04.21.537779\" xlink:type=\"simple\">https:\/\/doi.org\/10.1101\/2023.04.21.537779<\/jats:ext-link>\n            ) made an impact on his research and changed the way he thinks how functional genomics and high-throughput screens provide novel insights into pathogen pathogenesis.\n          <\/jats:p>","DOI":"10.1128\/msphere.00271-23","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T13:00:12Z","timestamp":1688043612000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["mSphere of Influence: Systemic decoding gene function in\n            <i>Toxoplasma gondii<\/i>\n            pathogenesis\u2014CRISPR screens and beyond"],"prefix":"10.1128","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9451-0041","authenticated-orcid":true,"given":"Yifan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School , Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.12.009"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.019"},{"key":"e_1_3_1_5_2","doi-asserted-by":"crossref","unstructured":"Butterworth S Kordova K Chandrasekaran S Thomas KK Torelli F Lockyer EJ Edwards A Goldstone R Koshy AA Treeck M\n. 2023. Mapping host-microbe transcriptional interactions by dual perturb-seq. bioRxiv. doi:10.1101\/2023.04.21.537779","DOI":"10.1101\/2023.04.21.537779"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18991-8"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01114-y"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00005-17"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00271-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00271-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T18:30:32Z","timestamp":1693506632000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msphere.00271-23"}},"subtitle":[],"editor":[{"given":"Ira J.","family":"Blader","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,8,24]]},"references-count":7,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,8,24]]}},"alternative-id":["10.1128\/msphere.00271-23"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00271-23","relation":{},"ISSN":["2379-5042"],"issn-type":[{"type":"electronic","value":"2379-5042"}],"subject":[],"published":{"date-parts":[[2023,8,24]]},"assertion":[{"value":"2023-05-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00271-23"}}