{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:43:14Z","timestamp":1791873794636,"version":"4.3.4"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000691","name":"Academy of Medical Sciences","doi-asserted-by":"crossref","award":["SBF006\\1142"],"award-info":[{"award-number":["SBF006\\1142"]}],"id":[{"id":"10.13039\/501100000691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100019690","name":"Medical Research Council Centre for Medical Mycology","doi-asserted-by":"crossref","award":["MR\/W502649\/1"],"award-info":[{"award-number":["MR\/W502649\/1"]}],"id":[{"id":"10.13039\/501100019690","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["102705 and 217163"],"award-info":[{"award-number":["102705 and 217163"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100019690","name":"Medical Research Council Centre for Medical Mycology","doi-asserted-by":"crossref","award":["MR\/N006364\/2 and MR\/V033417\/1"],"award-info":[{"award-number":["MR\/N006364\/2 and MR\/V033417\/1"]}],"id":[{"id":"10.13039\/501100019690","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2024,6,25]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The fungal pathogen\n              <jats:italic>Cryptococcus neoformans (C. neoformans<\/jats:italic>\n              ) forms yeast cells of different sizes and morphological characteristics during infection. These features are usually not seen in standard laboratory\n              <jats:italic>in vitro<\/jats:italic>\n              conditions. Here, we describe\n              <jats:italic>in vivo<\/jats:italic>\n              cell morphologies when\n              <jats:italic>C. neoformans<\/jats:italic>\n              is grown in human plasma-like medium at 37\u00b0C, 5% CO\n              <jats:sub>2<\/jats:sub>\n              . We observed mixed-size populations of cells less than 1 \u00b5m up to 16.8 \u00b5m in cell diameter, increased capsule size, high chitin, and DNA content in larger cells. Our findings show that serum is not required for human plasma-like medium (HPLM)-induced\n              <jats:italic>C. neoformans<\/jats:italic>\n              cellular heterogeneity. Thus, this new method offers an opportunity to investigate factors of\n              <jats:italic>C. neoformans<\/jats:italic>\n              that mediate pathogenesis or host\u2013pathogen interactions in a physiologically relevant setting.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                We provide a description of new\n                <jats:italic>in vitro<\/jats:italic>\n                culture condition using the human plasma-like medium that supports the formation of the full range of\n                <jats:italic>in vivo<\/jats:italic>\n                cell morphologies of\n                <jats:italic>C. neoformans<\/jats:italic>\n                .\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00281-24","type":"journal-article","created":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T13:01:11Z","timestamp":1716296471000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Human plasma-like medium (HPLM) induces\n            <i>Cryptococcus neoformans in vivo<\/i>\n            cell morphologies"],"prefix":"10.1128","volume":"9","author":[{"given":"Eduardo","family":"G. Mozo","sequence":"first","affiliation":[{"name":"Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orlando","family":"Ross","sequence":"additional","affiliation":[{"name":"Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raif","family":"Yuecel","sequence":"additional","affiliation":[{"name":"Faculty of Health and Life Sciences, Exeter Centre for Cytomics, The Bioeconomy Centre, University of Exeter, Exeter, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6556-6476","authenticated-orcid":false,"given":"Ivy M.","family":"Dambuza","sequence":"additional","affiliation":[{"name":"Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9645-8547","authenticated-orcid":true,"given":"Liliane","family":"Mukaremera","sequence":"additional","affiliation":[{"name":"Medical Research Council Centre for Medical Mycology, University of Exeter, Exeter, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000953"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000945"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2022.08.017"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006978"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006982"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007007"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.03.023"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.05179-11"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02509-21"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004701"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.150.3.1292-1296.1982"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.14.7.4912-4919.1994"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-8-2355"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02580-14"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2019.100759"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00281-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00281-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T13:02:43Z","timestamp":1719320563000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msphere.00281-24"}},"subtitle":[],"editor":[{"given":"Teresa R.","family":"O'Meara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,6,25]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6,25]]}},"alternative-id":["10.1128\/msphere.00281-24"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00281-24","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,25]]},"assertion":[{"value":"2024-04-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00281-24"}}