{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:08:50Z","timestamp":1767164930933,"version":"build-2238731810"},"reference-count":78,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T00:00:00Z","timestamp":1536710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,10,31]],"date-time":"2018-10-31T00:00:00Z","timestamp":1540944000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01DC013313"],"award-info":[{"award-number":["R01DC013313"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2018,10,31]]},"abstract":"<jats:p>\n                    Otitis media is the most common bacterial infection in childhood. Current therapies are limited in the prevention of chronic or recurrent otitis media which leads to increased antibiotic exposure and represents a significant socioeconomic burden. In this study, we delineate the effect of nutritional limitation on the intracellular trafficking pathways used by nontypeable\n                    <jats:named-content content-type=\"genus-species\">Haemophilus influenzae<\/jats:named-content>\n                    (NTHI). Moreover, transient limitation of heme-iron led to the development of intracellular bacterial communities that are known to contribute to persistence and recurrence in other diseases. New approaches for therapeutic interventions that reduce the production of intracellular bacterial communities and promote trafficking through the endolysosomal pathway were revealed through the use of pharmacological inhibition of macropinocytosis. This work demonstrates the importance of an intracellular niche for NTHI and provides new approaches for intervention for acute, chronic, and recurring episodes of otitis media.\n                  <\/jats:p>","DOI":"10.1128\/msphere.00286-18","type":"journal-article","created":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T09:31:13Z","timestamp":1536658273000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Transient Nutrient Deprivation Promotes Macropinocytosis-Dependent Intracellular Bacterial Community Development"],"prefix":"10.1128","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4624-1347","authenticated-orcid":false,"given":"Rachael L.","family":"Hardison","sequence":"first","affiliation":[{"name":"Center for Microbial Pathogenesis, The Research Institute at Nationwide Children\u2019s Hospital, Columbus, Ohio, USA"},{"name":"The Ohio State University College of Medicine, Columbus, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek R.","family":"Heimlich","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, The Research Institute at Nationwide Children\u2019s Hospital, Columbus, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alistair","family":"Harrison","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, The Research Institute at Nationwide Children\u2019s Hospital, Columbus, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wandy L.","family":"Beatty","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Rains","sequence":"additional","affiliation":[{"name":"Duke Proteomics and Metabolomics Core Facility, Duke Center for Genomic and Computational Biology, Duke University, Durham, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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