{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T22:26:57Z","timestamp":1773959217954,"version":"3.50.1"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T00:00:00Z","timestamp":1559692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI124723"],"award-info":[{"award-number":["AI124723"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R35 GM124976"],"award-info":[{"award-number":["R35 GM124976"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DK056645"],"award-info":[{"award-number":["R01 DK056645"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DK109714"],"award-info":[{"award-number":["R01 DK109714"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2019,6,26]]},"abstract":"<jats:p>\n                    <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n                    is a single-celled parasite that has infected up to one-third of the world\u2019s population. Significant overhauls in gene expression in both the parasite and the host cell accompany parasite invasion, and a better understanding of these changes may lead to the development of new therapeutic agents. In this study, we employed ribosome profiling to determine the changes that occur at the levels of transcription and translation in both the parasite and the infected host cell at the same time. We discovered features of\n                    <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n                    mRNAs that suggest a means for controlling parasite gene expression under stressful conditions. We also show that differences in host gene expression occur depending on whether they are confluent or not. Our findings demonstrate the feasibility of using ribosomal profiling to interrogate the host-parasite dynamic under a variety of conditions.\n                  <\/jats:p>","DOI":"10.1128\/msphere.00292-19","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T12:05:30Z","timestamp":1559649930000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Simultaneous Ribosome Profiling of Human Host Cells Infected with Toxoplasma gondii"],"prefix":"10.1128","volume":"4","author":[{"given":"Michael J.","family":"Holmes","sequence":"first","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana, USA"},{"name":"Department of Pharmacology &amp; Toxicology, Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Premal","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Genetics, Rutgers University, Piscataway, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald C.","family":"Wek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1823-8642","authenticated-orcid":false,"suffix":"Jr.","given":"William J.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Toxicology, Indiana University School of Medicine, Indianapolis, Indiana, USA"},{"name":"Department of Microbiology &amp; Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091014-104100"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-018-2808-x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2017.08.006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199448020-00005"},{"key":"e_1_3_2_6_2","first-page":"1","article-title":"Armed and dangerous: Toxoplasma gondii uses an arsenal of secretory proteins to infect host cells","volume":"48","author":"Carruthers VB","year":"1999","unstructured":"Carruthers VB. 1999. Armed and dangerous: Toxoplasma gondii uses an arsenal of secretory proteins to infect host cells. Parasitol Int 48:1\u201310.","journal-title":"Parasitol Int"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00005-17"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2018.56.2.135"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01305.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00244-18"},{"key":"e_1_3_2_11_2","article-title":"Regulation of arginine transport by GCN2 eIF2 kinase is important for replication of the intracellular parasite Toxoplasma gondii","author":"Augusto L","unstructured":"Augusto L, Amin P, Wek R, Sullivan W, Jr. Regulation of arginine transport by GCN2 eIF2 kinase is important for replication of the intracellular parasite Toxoplasma gondii. 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