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Abnormal expression of lncRNA is associated with uncontrolled cell growth in many forms of cancer. Recent studies have highlighted the role of lncRNAs in\n                      <jats:italic toggle=\"yes\">Aspergillus fumigatus<\/jats:italic>\n                      in azole stress response and virulence. Here, using a transcriptome data set of\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      response to stress, we identified\n                      <jats:italic toggle=\"yes\">afu-254<\/jats:italic>\n                      as an 854 bp lncRNA that plays a role in modulating oxidative stress, fungal sub-MIC azole response to posaconazole and itraconazole, cell wall stress, macrophage phagocytosis and killing\n                      <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                      , and virulence in an invertebrate model of\n                      <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                      infection. Importantly,\n                      <jats:italic toggle=\"yes\">afu-254<\/jats:italic>\n                      does not produce cross-azole susceptible responses and plays a role in fungal azole responses against posaconazole and itraconazole but not voriconazole. Furthermore, we showed that stoichiometric levels of\n                      <jats:italic toggle=\"yes\">afu-254<\/jats:italic>\n                      are important for its function, and ectopic overexpression of\n                      <jats:italic toggle=\"yes\">afu-254<\/jats:italic>\n                      in the WT strain leads to an antimorph. This phenotype may stem from a higher-order structure that is denatured with heat, indicating the presence of a non-functional isoform. In conclusion, we characterized a novel lncRNA,\n                      <jats:italic toggle=\"yes\">afu-254<\/jats:italic>\n                      , that is important for stress response and virulence in the pathogenic fungus\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      .\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Failure of azole treatment for invasive\n                        <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                        infection by both drug-resistant and drug-sensitive isolates is an area of concern and global importance. Fungal stress response is multifaceted, and long non-coding RNAs have emerged as important players in mediating it, including regulating responses to azole antifungals. Here, we have identified a long non-coding RNA,\n                        <jats:italic toggle=\"yes\">afu-254<\/jats:italic>\n                        , that plays a role in modulating fungal response to oxidative stress, cell wall stress, azole stress, immune cell stress, and virulence in an invertebrate model of invasive\n                        <jats:italic toggle=\"yes\">Aspergillus<\/jats:italic>\n                        infection.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00297-26","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T13:01:04Z","timestamp":1781010064000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["A long non-coding RNA,\n                    <i>afu-254<\/i>\n                    , is required for the oxidative stress response, cell wall stress response, azole susceptibility, and virulence in\n                    <i>Aspergillus fumigatus<\/i>"],"prefix":"10.1128","volume":"11","author":[{"given":"Ritu","family":"Devkota","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037s24f05","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Sciences, Clemson University","place":["Clemson, USA"]},{"id":[{"id":"https:\/\/ror.org\/037s24f05","id-type":"ROR","asserted-by":"publisher"}],"name":"Eukaryotic Pathogen Innovation Center, Clemson University","place":["Clemson, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nava Raj","family":"Poudyal","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037s24f05","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Sciences, Clemson University","place":["Clemson, USA"]},{"id":[{"id":"https:\/\/ror.org\/037s24f05","id-type":"ROR","asserted-by":"publisher"}],"name":"Eukaryotic Pathogen Innovation Center, Clemson University","place":["Clemson, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jazmin","family":"Reyes Servin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00d9ah105","id-type":"ROR","asserted-by":"publisher"}],"name":"Molecular Cell Biology Department, University of California Merced","place":["Merced, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julianna","family":"Lenz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00d9ah105","id-type":"ROR","asserted-by":"publisher"}],"name":"Molecular Cell Biology Department, University of California Merced","place":["Merced, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3631-2594","authenticated-orcid":true,"given":"Kelly M.","family":"Shepardson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00d9ah105","id-type":"ROR","asserted-by":"publisher"}],"name":"Molecular Cell Biology Department, University of California Merced","place":["Merced, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7202-8203","authenticated-orcid":true,"given":"Sourabh","family":"Dhingra","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037s24f05","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Sciences, Clemson University","place":["Clemson, USA"]},{"id":[{"id":"https:\/\/ror.org\/037s24f05","id-type":"ROR","asserted-by":"publisher"}],"name":"Eukaryotic Pathogen Innovation Center, Clemson University","place":["Clemson, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2023.2172264"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofw036"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanwpc.2023.100888"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw326"},{"key":"e_1_3_3_6_2","unstructured":"World Health Organization. 2025. 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