{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:13:21Z","timestamp":1753881201525,"version":"3.41.2"},"reference-count":5,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T00:00:00Z","timestamp":1562112000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T00:00:00Z","timestamp":1566950400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2019,8,28]]},"abstract":"<jats:p>\n            Alfredo J. Guerra works in the field of molecular parasitology and structural biology. In this mSphere of Influence article, he reflects on how \u201cEfficient Gene Disruption in Diverse Strains of\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            Using CRISPR\/CAS9\u201d by Bang Shen et al. (mBio 5:e01114-14, 2014,\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1128\/mBio.01114-14\">https:\/\/doi.org\/10.1128\/mBio.01114-14<\/jats:ext-link>\n            ) and \u201cEfficient Genome Engineering of\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            using CRISPR\/CAS9\u201d by Saima M. Sidik et al. (PLoS One 9:e100450, 2014,\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1371\/journal.pone.0100450\">https:\/\/doi.org\/10.1371\/journal.pone.0100450<\/jats:ext-link>\n            ) made an impact on him by successfully implementing strategies to genetically manipulate\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            using CRISPR\/CAS9 gene editing technology.\n          <\/jats:p>","DOI":"10.1128\/msphere.00307-19","type":"journal-article","created":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T10:32:16Z","timestamp":1562063536000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["mSphere of Influence: Ushering in the CRISPR Revolution to\n            <i>Toxoplasma<\/i>\n            Biology"],"prefix":"10.1128","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8764-8413","authenticated-orcid":false,"given":"Alfredo J.","family":"Guerra","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01114-14"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100450"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.019"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm981"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007476"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00307-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00307-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T04:22:42Z","timestamp":1646713362000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00307-19"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,28]]},"references-count":5,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,8,28]]}},"alternative-id":["10.1128\/mSphere.00307-19"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00307-19","relation":{},"ISSN":["2379-5042"],"issn-type":[{"type":"electronic","value":"2379-5042"}],"subject":[],"published":{"date-parts":[[2019,8,28]]},"assertion":[{"value":"2019-07-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00307-19"}}