{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:50:39Z","timestamp":1788328239250,"version":"build-2803163510"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["5R01AI146103"],"award-info":[{"award-number":["5R01AI146103"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["7R21AI144878"],"award-info":[{"award-number":["7R21AI144878"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007699","name":"University of Georgia","doi-asserted-by":"publisher","award":["Distinguished Research Professorship"],"award-info":[{"award-number":["Distinguished Research Professorship"]}],"id":[{"id":"10.13039\/100007699","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,7,29]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                      , a fungal commensal and pathogen, occupies diverse niches in the human host. Its broad metabolic repertoire is critical for its survival. The model yeast\n                      <jats:italic toggle=\"yes\">Saccharomyces cerevisiae<\/jats:italic>\n                      provides a starting point for analysis of\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      physiology and regulatory circuitry, but there are many examples of rewired transcription factors that govern different processes in the two organisms. We focus here on Adr1, which in\n                      <jats:italic toggle=\"yes\">S. cerevisiae<\/jats:italic>\n                      promotes alternative carbon source utilization and in\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      promotes ergosterol synthesis. We find that\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      Adr1 is also required for growth on citrate and compounds that feed into the citric acid cycle, like glutamate and malate. RNA-sequencing (RNA-seq) shows that predicted citrate metabolic genes, representing both the citric acid cycle and gluconeogenesis, are downregulated in an\n                      <jats:italic toggle=\"yes\">adr1<\/jats:italic>\n                      \u0394\/\u0394 mutant. In fact, the three Adr1-dependent genes\n                      <jats:italic toggle=\"yes\">HGT17, MDH1,<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">PCK1<\/jats:italic>\n                      are required for growth on citrate, as indicated by deletion mutant phenotypes. The hyphal regulator\n                      <jats:italic toggle=\"yes\">EED1<\/jats:italic>\n                      has a negative role in citrate utilization, and an\n                      <jats:italic toggle=\"yes\">adr1<\/jats:italic>\n                      \u0394\/\u0394\n                      <jats:italic toggle=\"yes\">eed1<\/jats:italic>\n                      \u0394\/\u0394 double mutant is defective for growth on citrate. This result argues that Adr1 acts downstream or independently of Eed1 to govern citrate utilization.\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      Adr1 is rewired compared to its\n                      <jats:italic toggle=\"yes\">S. cerevisiae<\/jats:italic>\n                      ortholog to govern the ability to use citrate, which\n                      <jats:italic toggle=\"yes\">S. cerevisiae<\/jats:italic>\n                      lacks, and potentially to respond to Eed1, for which\n                      <jats:italic toggle=\"yes\">S. cerevisiae<\/jats:italic>\n                      lacks an ortholog.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                        is a major fungal pathogen of humans, and its ability to grow on a range of carbon sources is critical for pathogenicity. Here, we find that a known regulator of ergosterol synthesis, Adr1, is also required to use citrate as a carbon source. Adr1 acts downstream or independently of Eed1, a well-known regulator of hypha formation and citrate utilization.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00311-25","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T09:00:07Z","timestamp":1749632407000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Control of citrate utilization by\n                    <i>Candida albicans<\/i>\n                    Adr1"],"prefix":"10.1128","volume":"10","author":[{"given":"Amelia M.","family":"White","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00te3t702","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, University of Georgia","place":["Athens, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0868-4000","authenticated-orcid":true,"given":"Aaron P.","family":"Mitchell","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00te3t702","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, University of Georgia","place":["Athens, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.07.001"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.157"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-06"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00372-06"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005566"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2021.10.003"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-01-0002"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00099"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11010005"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00206-09"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008582"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000612"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00676.x"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.5.1076-1087.2004"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/320283a0"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01807-23"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-23-1-65"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1062"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.10.4419-4424.1999"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.174623.114"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007787"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05759.x"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-7-230"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.605"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyaf001"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac963"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-002-2330-4"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.07003.x"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01009.x"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24095-8"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","unstructured":"Reyes-Chavez B Kerkaert JD Huberman LB. 2025. 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