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However, the antifungal activities and molecular responses of fungi to different mVCs remain largely undescribed. In this study, we first evaluated the responses of pathogenic fungi to treatment with mVCs from\n            <jats:italic>Paenarthrobacter ureafaciens<\/jats:italic>\n            . Then, we utilized the well-characterized fungal model organism\n            <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n            to study the potential mechanistic effects of the mVCs. Our data showed that exposure to\n            <jats:italic>P. ureafaciens<\/jats:italic>\n            mVCs leads to reduced growth of several pathogenic fungi, and in yeast cells, mVC exposure prompts the accumulation of reactive oxygen species. Further experiments with\n            <jats:italic>S. cerevisiae<\/jats:italic>\n            deletion mutants indicated that Slt2\/Mpk1 and Hog1 MAPKs play major roles in the yeast response to\n            <jats:italic>P. ureafaciens<\/jats:italic>\n            mVCs. Transcriptomic analysis revealed that exposure to mVCs was associated with 1,030 differentially expressed genes (DEGs) in yeast. According to gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses, many of these DEGs are involved in mitochondrial dysfunction, cell integrity, mitophagy, cellular metabolism, and iron uptake. Genes encoding antimicrobial proteins were also significantly altered in the yeast after exposure to mVCs. These findings suggest that oxidative damage and mitochondrial dysfunction are major contributors to the fungal toxicity of mVCs. Furthermore, our data showed that cell wall, antioxidant, and antimicrobial defenses are induced in yeast exposed to mVCs. Thus, our findings expand upon previous research by delineating the transcriptional responses of the fungal model.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Since the use of bacteria-emitted volatile compounds in phytopathogen control is of considerable interest, it is important to understand the molecular mechanisms by which fungi may adapt to microbial volatile compounds (mVCs).\n              <jats:italic>Paenarthrobacter ureafaciens<\/jats:italic>\n              is an isolated bacterium from disease-suppressive soil that belongs to the\n              <jats:italic>Actinomycetota<\/jats:italic>\n              phylum.\n              <jats:italic>P. ureafaciens<\/jats:italic>\n              mVCs showed a potent antifungal effect on phytopathogens, which may contribute to disease suppression in soil. However, our knowledge about the antifungal mechanism of mVCs is limited. This study has proven that mVCs are toxic to fungi due to oxidative stress and mitochondrial dysfunction. To deal with mVC toxicity, antioxidants and physical defenses are required. Furthermore, iron uptake and CAP proteins are required for antimicrobial defense, which is necessary for fungi to deal with the thread from mVCs. This study provides essential foundational knowledge regarding the molecular responses of fungi to inhibitory mVCs.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00324-23","type":"journal-article","created":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T13:05:27Z","timestamp":1695733527000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Antifungal mechanism of volatile compounds emitted by\n            <i>Actinomycetota Paenarthrobacter ureafaciens<\/i>\n            from a disease-suppressive soil on\n            <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1128","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1650-0953","authenticated-orcid":false,"given":"Tri-Phuong","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Life Sciences, National Cheng Kung University , Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Rui","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Cheng Kung University , Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Han","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Program in Translational Agricultural Sciences, National Cheng Kung University and Academia Sinica , Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Yu","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Cheng Kung University , Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chieh-An","family":"Ou","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Cheng Kung University , Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping-Fu","family":"Hou","sequence":"additional","affiliation":[{"name":"Kaohsiung District Agricultural Research and Extension Station , Pingtung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huang-Mo","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Cheng Kung University , Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Hung","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Cheng Kung University , Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Ohme-Takagi","sequence":"additional","affiliation":[{"name":"Institute of Tropical Plant Sciences and Microbiology, National Cheng Kung University , Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7459-162X","authenticated-orcid":false,"given":"Hao-Jen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Cheng Kung University , Tainan, Taiwan"},{"name":"Graduate Program in Translational Agricultural Sciences, National Cheng Kung University and Academia Sinica , Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf403200g"},{"key":"e_1_3_4_3_2","first-page":"11183","volume-title":"Proceedings of the National Academy of Sciences","volume":"107","author":"Le\u00e3o PN","year":"2010","unstructured":"Le\u00e3o PN , Pereira AR , Liu W-T , Ng J , Pevzner PA , Dorrestein PC , K\u00f6nig GM , Vasconcelos VM , Gerwick WH. 2010. 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