{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:42:48Z","timestamp":1791852168318,"version":"4.3.4"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T00:00:00Z","timestamp":1629849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T00:00:00Z","timestamp":1629849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19fk0108035"],"award-info":[{"award-number":["JP19fk0108035"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19fk0108036"],"award-info":[{"award-number":["JP19fk0108036"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19fk0108053"],"award-info":[{"award-number":["JP19fk0108053"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20fk0108123"],"award-info":[{"award-number":["JP20fk0108123"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP18fm0108003"],"award-info":[{"award-number":["JP18fm0108003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20gm0910005"],"award-info":[{"award-number":["JP20gm0910005"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP16K08362"],"award-info":[{"award-number":["JP16K08362"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP17H04003"],"award-info":[{"award-number":["JP17H04003"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP19fm0108003"],"award-info":[{"award-number":["JP19fm0108003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20wm0125010"],"award-info":[{"award-number":["JP20wm0125010"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2021,8,25]]},"abstract":"<jats:p>Neutralization tests are the most reliable assay for flavivirus antibody detection; however, these assays are not suitable for high-throughput processing due to their time-consuming and labor-intensive nature. In this study, we developed single-round infectious particles (SRIPs) with a luciferase gene for dengue virus types 1 to 4, Japanese encephalitis virus, and Zika virus for use in a safe, high-throughput neutralization assay.<\/jats:p>","DOI":"10.1128\/msphere.00339-21","type":"journal-article","created":{"date-parts":[[2021,7,14]],"date-time":"2021-07-14T15:59:03Z","timestamp":1626278343000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012"],"prefix":"10.1128","volume":"6","author":[{"given":"Atsushi","family":"Yamanaka","sequence":"first","affiliation":[{"name":"Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Mahidol-Osaka Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mami","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan"},{"name":"Department of Laboratory Medicine, The Jikei University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamaki","family":"Okabayashi","sequence":"additional","affiliation":[{"name":"Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pannamthip","family":"Pitaksajjakul","sequence":"additional","affiliation":[{"name":"Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Center of Excellence for Antibody Research (CEAR), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pongrama","family":"Ramasoota","sequence":"additional","affiliation":[{"name":"Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Center of Excellence for Antibody Research (CEAR), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayoshi","family":"Fukasawa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Hanada","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomokazu","family":"Matsuura","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Jikei University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0153-3533","authenticated-orcid":true,"given":"Masamichi","family":"Muramatsu","sequence":"additional","affiliation":[{"name":"Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuo","family":"Shioda","sequence":"additional","affiliation":[{"name":"Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0296-8843","authenticated-orcid":true,"given":"Ryosuke","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan"},{"name":"Department of Biological Science and Technology, Tokyo University of Science, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Flaviviridae.","author":"Lindenbach BD","year":"2013","unstructured":"Lindenbach BD, Murray CL, Thiel H-J, Rice CM. 2013. Flaviviridae. Lippincott William & Wilkins, Philadelphia, PA."},{"key":"e_1_3_2_3_2","first-page":"747","volume-title":"Fields virology","author":"Pierson TC","year":"2013","unstructured":"Pierson TC, Diamond MS. 2013. Flaviviruses, p 747\u2013794. In Fields virology, 6th ed. Lippincott Williams & Wilkins, Philadelphia, PA.","edition":"6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"Wilder-Smith A Osman S. 2020. Public health emergencies of international concern: a historic overview. J Travel Med 27:taaa227. doi:10.1093\/jtm\/taaa227.","DOI":"10.1093\/jtm\/taaa227"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4379-3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-35643-6"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0022"},{"key":"e_1_3_2_8_2","first-page":"267","article-title":"Studies on an epidemic of Japanese encephalitis in the northern region of Thailand in 1969 and 1970","volume":"14","author":"Yamada T","year":"1971","unstructured":"Yamada T, Rojanasuphot S, Takagi M, Wungkobkiat S, Hirota T. 1971. Studies on an epidemic of Japanese encephalitis in the northern region of Thailand in 1969 and 1970. Biken J 14:267\u2013296.","journal-title":"Biken J"},{"key":"e_1_3_2_9_2","first-page":"625","article-title":"Japanese encephalitis: current worldwide status","volume":"63","author":"Umenai T","year":"1985","unstructured":"Umenai T, Krzysko R, Bektimirov TA, Assaad FA. 1985. Japanese encephalitis: current worldwide status. Bull World Health Organ 63:625\u2013631.","journal-title":"Bull World Health Organ"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2010.03.022"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.10.083"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.ackd.2019.01.002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"291","DOI":"10.4049\/jimmunol.99.2.291","article-title":"Dengue virus identification by the plaque reduction neutralization test","volume":"99","author":"Russell PK","year":"1967","unstructured":"Russell PK, Nisalak A. 1967. Dengue virus identification by the plaque reduction neutralization test. J Immunol 99:291\u2013296.","journal-title":"J Immunol"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.06.017"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-34865-y"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2017.02.011"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.058008-0"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003241"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001876"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy164"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.2147\/TCRM.S51168"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.02.004"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30168-2"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00339-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00339-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T05:24:36Z","timestamp":1672809876000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00339-21"}},"subtitle":[],"editor":[{"given":"Shirit","family":"Einav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,8,25]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,8,25]]}},"alternative-id":["10.1128\/mSphere.00339-21"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00339-21","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,25]]},"assertion":[{"value":"2021-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00339-21"}}