{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:19:10Z","timestamp":1787771950340,"version":"build-2784847793"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI173270"],"award-info":[{"award-number":["R01AI173270"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,9,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The structural role of \u03b2-1,6-glucan has remained under-investigated in filamentous fungi compared to other fungal cell wall polymers, and previous studies have shown that the cell wall of the mycelium of\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      did not contain \u03b2-1,6-glucans. In contrast, the current solid-state NMR investigations showed that the conidial cell wall contained a low amount of \u03b2-1,6-glucan. ssNMR comparisons of the\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      \u03b2-1,6-glucans showed they are structurally similar. The deletion of the\n                      <jats:italic toggle=\"yes\">KRE6<\/jats:italic>\n                      gene which is the only\n                      <jats:italic toggle=\"yes\">KRE<\/jats:italic>\n                      gene in the\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      genome resulted in a mutant depleted of \u03b2-1,6-glucan which has a growth phenotype similar to the parental strain. Even though it is not an essential polymer in\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      , \u03b2-1,6-glucan plays a role in cell wall organization since the\n                      <jats:italic toggle=\"yes\">kre6<\/jats:italic>\n                      \u0394 mutant showed a higher sensitivity to Congo-red and Calcofluor white which are known to be general cell wall inhibitors. It is also another example of the significant structural differences seen between conidium and mycelium of filamentous fungi.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study shows for the first time that \u03b2-1,6-glucans are found in\n                        <jats:italic toggle=\"yes\">Aspergillus fumigatus<\/jats:italic>\n                        . Interestingly, this polysaccharide was specifically identified in conidia.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00341-25","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T13:40:57Z","timestamp":1757338857000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Kre6-dependent \u03b2-1,6-glucan biosynthesis only occurs in the conidium of\n                    <i>Aspergillus fumigatus<\/i>"],"prefix":"10.1128","volume":"10","author":[{"given":"Kalpana","family":"Singh","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05hs6h993","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Michigan State University","place":["East Lansing, 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