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Although destroyed by pasteurization,<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>is among the most heat-resistant non-spore-forming bacteria. This poses a risk to food safety, as listeriosis is commonly associated with ready-to-eat foods that are consumed without thorough heating. However,<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>strains differ in their ability to survive high temperatures, and comprehensive understanding of the genetic mechanisms underlying these differences is still limited. Whole-genome-sequence analysis and phenotypic characterization allowed us to identify a novel plasmid, designated pLM58, and a plasmid-borne ATP-dependent protease (ClpL), which mediated heat resistance in<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>. As the first report on plasmid-mediated heat resistance in<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>, our study sheds light on the accessory genetic mechanisms rendering certain<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>strains particularly capable of surviving high temperatures\u2014with plasmid-borne ClpL being a potential predictor of elevated heat resistance.<\/jats:p>","DOI":"10.1128\/msphere.00364-17","type":"journal-article","created":{"date-parts":[[2017,11,2]],"date-time":"2017-11-02T00:25:33Z","timestamp":1509582333000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Heat Resistance Mediated by pLM58 Plasmid-Borne ClpL in Listeria monocytogenes"],"prefix":"10.1128","volume":"2","author":[{"given":"Anna","family":"P\u00f6ntinen","sequence":"first","affiliation":[{"name":"Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariella","family":"Aalto-Araneda","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miia","family":"Lindstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannu","family":"Korkeala","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/br.30.2.309-382.1966"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198301273080407"},{"key":"e_1_3_2_4_2","first-page":"328","article-title":"Incidence and trends of infection with pathogens transmitted commonly through food \u2013 Foodborne Diseases Active Surveillance Network, 10 U.S. sites, 2006\u20132013","volume":"63","author":"Crim SM","year":"2014","unstructured":"Crim SM Iwamoto M Huang JY Griffin PM Gilliss D Cronquist AB Cartter M Tobin-D\u2019Angelo M Blythe D Smith K Lathrop S Zansky S Cieslak PR Dunn J Holt KG Lance S Tauxe R Henao OL Centers for Disease Control and Prevention (CDC) . 2014. 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