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Caspofungin is an echinocandin that impacts the construction of the fungal cell wall by inhibiting the biosynthesis of the \u03b2-1,3-glucan polysaccharide. Caspofungin is a fungistatic drug and is recommended as a second-line therapy for treatment of aspergillosis. Treatment at high concentrations induces an increase of fungal growth, a phenomenon called the<jats:underline>c<\/jats:underline>aspofungin<jats:underline>p<\/jats:underline>aradoxical<jats:underline>e<\/jats:underline>ffect (CPE). Collaboration between the mitogen-activated protein kinases (MAPK) of the cell wall integrity (MapkA) and high-osmolarity glycerol (SakA) pathways is essential for CPE. Here, we investigate the global proteome and phosphoproteome of<jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>wild-type, \u0394<jats:italic>mpkA<\/jats:italic>, and \u0394<jats:italic>sakA<\/jats:italic>strains upon CPE. This study showed intense cross talk between the two MAPKs for the CPE and identified novel protein kinases and transcription factors possibly important for CPE. Increased understanding of how the modulation of protein phosphorylation may affect the fungal growth in the presence of caspofungin represents an important step in the development of new strategies and methods to combat the fungus inside the host.<\/jats:p>","DOI":"10.1128\/msphere.00365-20","type":"journal-article","created":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T14:17:07Z","timestamp":1590502627000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Phosphoproteomics of Aspergillus fumigatus Exposed to the Antifungal Drug Caspofungin"],"prefix":"10.1128","volume":"5","author":[{"given":"Eliciane Cevolani","family":"Mattos","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, 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