{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T08:51:35Z","timestamp":1778835095337,"version":"3.51.4"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T00:00:00Z","timestamp":1485302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T00:00:00Z","timestamp":1487721600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100001250","name":"Morris Animal Foundation","doi-asserted-by":"publisher","award":["D08ZO-316"],"award-info":[{"award-number":["D08ZO-316"]}],"id":[{"id":"10.13039\/100001250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2017,2,22]]},"abstract":"<jats:p>\n            CWD is the only known TSE that affects free-ranging wildlife, specifically cervids such as elk, deer, moose, caribou, and reindeer. CWD has become endemic in both free-ranging and captive herds in North America, South Korea, and, most recently, northern Europe. The prion research community continues to debate the origins of CWD. Original foci of CWD emergence in Colorado and Wyoming coincident with the sheep TSE scrapie suggest that scrapie prions may have adapted to cervids to cause CWD. However, emerging evidence supports the idea that cervid PrP\n            <jats:sup>C<\/jats:sup>\n            may be more prone to misfolding to the pathological isoform. Here we test the hypothesis that cervid PrP\n            <jats:sup>C<\/jats:sup>\n            can spontaneously misfold to create\n            <jats:italic>de novo<\/jats:italic>\n            prions. Whether CWD can arise spontaneously as a sporadic TSE or represents a new TSE caused by cervid-adapted scrapie prions profoundly impacts surveillance and mitigation strategies.\n          <\/jats:p>","DOI":"10.1128\/msphere.00372-16","type":"journal-article","created":{"date-parts":[[2017,1,26]],"date-time":"2017-01-26T01:12:22Z","timestamp":1485393142000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["<i>De Novo<\/i>\n            Generation of a Unique Cervid Prion Strain Using Protein Misfolding Cyclic Amplification"],"prefix":"10.1128","volume":"2","author":[{"given":"Crystal","family":"Meyerett-Reid","sequence":"first","affiliation":[{"name":"Prion Research Center at Colorado State University, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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