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The agent causing brucellosis resides inside host cells within vacuoles termed\n            <jats:italic>Brucella-<\/jats:italic>\n            containing vacuoles (BCVs). Although a few host components required to escape the degradative lysosomal pathway and to establish the ER-derived replicative BCV (rBCV) have already been identified, the global understanding of this highly coordinated process is still partial, and many factors remain unknown. To gain deeper insight into these fundamental questions, we performed a genomewide RNA interference (RNAi) screen aiming at discovering novel host factors involved in the\n            <jats:italic>Brucella<\/jats:italic>\n            intracellular cycle. We identified 425 host proteins that contribute to\n            <jats:italic>Brucella<\/jats:italic>\n            cellular entry, intracellular trafficking, and replication. Together, this study sheds light on previously unknown host pathways required for the\n            <jats:italic>Brucella<\/jats:italic>\n            infection cycle and highlights the VPS retromer components as critical factors for the establishment of the\n            <jats:italic>Brucella<\/jats:italic>\n            intracellular replicative niche.\n          <\/jats:p>","DOI":"10.1128\/msphere.00380-19","type":"journal-article","created":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T11:48:59Z","timestamp":1561463339000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["A Role for the VPS Retromer in\n            <i>Brucella<\/i>\n            Intracellular Replication Revealed by Genomewide siRNA Screening"],"prefix":"10.1128","volume":"4","author":[{"given":"Alain","family":"Casanova","sequence":"first","affiliation":[{"name":"Biozentrum, University of Basel, Basel, 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