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Understanding the roles of sterols will facilitate the development of new drugs and counter drug resistance. LSO is required for the formation of the C-5\u2013C-6 double bond in the sterol core structure in mammals, fungi, protozoans, plants, and algae. Functions of this C-5\u2013C-6 double bond are not well understood. In this study, we generated and characterized a lathosterol oxidase-null mutant in\n                    <jats:named-content content-type=\"genus-species\">Leishmania major<\/jats:named-content>\n                    . Our data suggest that LSO is vital for the structure and membrane-stabilizing functions of leishmanial sterols. In addition, our results imply that while mutations in lathosterol oxidase can confer resistance to amphotericin B, an important antifungal and antiprotozoal agent, the alteration in sterol structure leads to significant defects in stress response that could be exploited for drug development.\n                  <\/jats:p>","DOI":"10.1128\/msphere.00380-20","type":"journal-article","created":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T09:10:22Z","timestamp":1593594622000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Lathosterol Oxidase (Sterol C-5 Desaturase) Deletion Confers Resistance to Amphotericin B and Sensitivity to Acidic Stress in Leishmania major"],"prefix":"10.1128","volume":"5","author":[{"given":"Yu","family":"Ning","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Texas Tech University, Lubbock, Texas, 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