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In addition to functioning as a protective barrier, the plasma membrane plays dynamic roles in a wide range of functions needed for virulence including nutrient uptake, cell wall synthesis, morphogenesis, resistance to stress, and invasive hyphal growth. Screening a collection of\n              <jats:italic>C. albicans<\/jats:italic>\n              mutants identified an understudied gene that is important for invasive hyphal growth, which we have termed\n              <jats:italic>CWR1<\/jats:italic>\n              (Cell Wall Regulatory kinase). A mutant strain lacking\n              <jats:italic>CWR1<\/jats:italic>\n              displayed defects in resisting stressful conditions that exacerbate cell wall defects. The Cwr1 protein shows strong similarity to protein kinases, suggesting it plays a regulatory role in coordinating plasma membrane and cell wall functions. A Cwr1\u2013green fluorescent protein (GFP) fusion protein localized to punctate patches associated with the plasma membrane that partially overlapped Membrane Compartment of Can1 (MCC)\/eisosome domains. In contrast to the static MCC\/eisosome domains, the Cwr1\u2013GFP patches were very dynamic. Truncation mutants lacking C-terminal sequences distal to the protein kinase domain failed to show detectable localization at the plasma membrane. Surprisingly, these mutant strains did not show the defects of a\n              <jats:italic>cwr1\u0394<\/jats:italic>\n              mutant, suggesting that localization to punctate patches associated with the plasma membrane is not essential for Cwr1 function. Altogether, these data indicate that Cwr1 contributes to the regulation of plasma membrane functions that promote proper morphogenesis and resistance to cell wall stress, both of which are important for\n              <jats:italic>C. albicans<\/jats:italic>\n              virulence.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              The ability of\n              <jats:italic>Candida albicans<\/jats:italic>\n              to grow invasively in the host and resist stress is critical for it to be an effective human pathogen. Identifying the genes that promote these processes is important for developing new strategies to block infection. Therefore, genetic methods were used in this study to identify a novel gene that is needed for invasive growth and stress resistance (Cell Wall Regulatory kinase [\n              <jats:italic>CWR1<\/jats:italic>\n              ]). Interestingly, the Cwr1 protein localized to punctate patches in the plasma membrane, some of which co-localized with specialized subdomains of the plasma membrane known as eisosomes that are known to promote stress resistance and invasive growth in the host. Thus, these studies identified a novel regulator of traits that are critical for\n              <jats:italic>C. albicans<\/jats:italic>\n              pathogenesis.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00391-24","type":"journal-article","created":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T15:51:18Z","timestamp":1732895478000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["The Cwr1 protein kinase localizes to the plasma membrane and mediates resistance to cell wall stress in\n            <i>Candida albicans<\/i>"],"prefix":"10.1128","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2774-2176","authenticated-orcid":false,"given":"Shamoon","family":"Naseem","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Stony Brook University","place":["Stony Brook, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8332-9789","authenticated-orcid":true,"given":"Jakub","family":"Zahumensk\u00fd","sequence":"additional","affiliation":[{"name":"Department of Functional Organization of Biomembranes, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic","place":["Prague, Czechia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla E.","family":"Lanze","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stony Brook University","place":["Stony Brook, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lois M.","family":"Douglas","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stony Brook University","place":["Stony Brook, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1433-0989","authenticated-orcid":true,"given":"Jan","family":"Mal\u00ednsk\u00fd","sequence":"additional","affiliation":[{"name":"Department of Functional Organization of Biomembranes, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic","place":["Prague, Czechia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5989-4086","authenticated-orcid":true,"given":"James B.","family":"Konopka","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stony Brook University","place":["Stony Brook, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"doi-asserted-by":"publisher","key":"e_1_3_3_2_2","DOI":"10.1126\/scitranslmed.3004404"},{"doi-asserted-by":"publisher","key":"e_1_3_3_3_2","DOI":"10.1056\/NEJMra1315399"},{"doi-asserted-by":"publisher","key":"e_1_3_3_4_2","DOI":"10.1016\/j.tim.2017.05.003"},{"doi-asserted-by":"publisher","key":"e_1_3_3_5_2","DOI":"10.3390\/biom5010142"},{"doi-asserted-by":"publisher","key":"e_1_3_3_6_2","DOI":"10.1016\/j.mib.2009.06.007"},{"doi-asserted-by":"publisher","key":"e_1_3_3_7_2","DOI":"10.3390\/pathogens10070859"},{"doi-asserted-by":"publisher","key":"e_1_3_3_8_2","DOI":"10.1128\/mBio.02503-19"},{"doi-asserted-by":"publisher","key":"e_1_3_3_9_2","DOI":"10.1038\/nrmicro.2017.107"},{"doi-asserted-by":"publisher","key":"e_1_3_3_10_2","DOI":"10.12688\/f1000research.18546.1"},{"doi-asserted-by":"publisher","key":"e_1_3_3_11_2","DOI":"10.1111\/cmi.12369"},{"doi-asserted-by":"publisher","key":"e_1_3_3_12_2","DOI":"10.1128\/EC.00106-15"},{"doi-asserted-by":"publisher","key":"e_1_3_3_13_2","DOI":"10.1128\/MMBR.00063-19"},{"doi-asserted-by":"publisher","key":"e_1_3_3_14_2","DOI":"10.1371\/journal.pgen.1007911"},{"doi-asserted-by":"publisher","key":"e_1_3_3_15_2","DOI":"10.1128\/mBio.00254-11"},{"doi-asserted-by":"publisher","key":"e_1_3_3_16_2","DOI":"10.1091\/mbc.E23-08-0324"},{"doi-asserted-by":"publisher","key":"e_1_3_3_17_2","DOI":"10.1091\/mbc.E16-01-0065"},{"doi-asserted-by":"publisher","key":"e_1_3_3_18_2","DOI":"10.1091\/mbc.e08-05-0479"},{"doi-asserted-by":"publisher","key":"e_1_3_3_19_2","DOI":"10.1242\/jcs.051227"},{"doi-asserted-by":"publisher","key":"e_1_3_3_20_2","DOI":"10.1038\/nature04472"},{"doi-asserted-by":"publisher","key":"e_1_3_3_21_2","DOI":"10.1091\/mbc.e03-04-0221"},{"doi-asserted-by":"publisher","key":"e_1_3_3_22_2","DOI":"10.1038\/nsmb.2080"},{"doi-asserted-by":"publisher","key":"e_1_3_3_23_2","DOI":"10.1091\/mbc.E10-12-1021"},{"doi-asserted-by":"publisher","unstructured":"Kefauver JM Hakala M Zou L Alba J Espadas J Tettamanti MG Estrozi LF Vanni S Roux A Desfosses A Loewith R. 2023. 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