{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:19:35Z","timestamp":1787620775276,"version":"build-2736575974"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2024,9,25]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Previously, we demonstrated that the majority of vancomycin-resistant\n                      <jats:italic>Enterococcus faecium<\/jats:italic>\n                      (VREfm) strains from in-patients of the University Hospital Erlangen, Germany, belonged to only three clonal lineages, namely ST117\/CT71\n                      <jats:italic>vanB<\/jats:italic>\n                      and two novel ST1299\n                      <jats:italic>vanA<\/jats:italic>\n                      lineages classified as CT3109 and CT1903. The goal of the current study was (i) to investigate whether VREfm is also detectable in wastewater of the city of Erlangen, (ii) to identify their molecular features, and (iii) to clarify whether VREfm could arise from the community of the city of Erlangen or can be (directly) connected to nosocomial infections in the hospital setting. From April to May 2023, a total of 244 VREfm strains from raw wastewater of the city of Erlangen were analyzed by core genome multilocus sequence typing (cgMLST). Moreover, 20 of them were further investigated for single nucleotide polymorphisms (SNPs). The molecular characterization of the wastewater VREfm strains revealed a high prevalence (27.9%) of the recently identified clonal lineage ST1299\/CT3109\n                      <jats:italic>vanA,<\/jats:italic>\n                      which is mainly characterized by the presence of the tetracycline-resistance determinant\n                      <jats:italic>tet(M<\/jats:italic>\n                      ) and the virulence genes\n                      <jats:italic>pilA<\/jats:italic>\n                      and\n                      <jats:italic>prpA<\/jats:italic>\n                      . The SNPs analysis revealed the presence of two major clusters, namely cluster I (\u226465 SNPs), which included well-known hospital-adapted\n                      <jats:italic>vanB<\/jats:italic>\n                      clonal lineages such as ST117\/CT71 and ST80\/CT1065 and cluster II (\u226470 SNPs), which were mainly characterized by the lineage ST1299\/CT3109\n                      <jats:italic>vanA<\/jats:italic>\n                      . Based on the concomitant resistance to vancomycin and tetracycline, we propose that ST1299\/CT3109\n                      <jats:italic>vanA<\/jats:italic>\n                      primarily originated and spread outside of hospital settings.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study provides a detailed genomic analysis of vancomycin-resistant\n                        <jats:italic>Enterococcus faecium<\/jats:italic>\n                        (VREfm) strains isolated from municipal wastewater with a particular focus on clonal lineages, antimicrobial resistance, and the presence of virulence genes. The high wastewater prevalence of the recently identified clonal lineage ST1299\/CT3109\n                        <jats:italic>vanA<\/jats:italic>\n                        , which has been previously detected in hospitals, suggests an enormous potential for future spread in Germany.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00396-24","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T09:01:36Z","timestamp":1724749296000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["High prevalence of the recently identified clonal lineage ST1299\/CT3109\n                    <i>vanA<\/i>\n                    among vancomycin-resistant\n                    <i>Enterococcus faecium<\/i>\n                    strains isolated from municipal wastewater"],"prefix":"10.1128","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9342-4388","authenticated-orcid":false,"given":"Giuseppe","family":"Valenza","sequence":"first","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander-Universit\u00e4t (FAU) Erlangen-N\u00fcrnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Eisenberger","sequence":"additional","affiliation":[{"name":"Bayerisches Landesamt f\u00fcr Gesundheit und Lebensmittelsicherheit, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Esse","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander-Universit\u00e4t (FAU) Erlangen-N\u00fcrnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1130-9727","authenticated-orcid":false,"given":"J\u00fcrgen","family":"Held","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander-Universit\u00e4t (FAU) Erlangen-N\u00fcrnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Verena","family":"Lehner-Reindl","sequence":"additional","affiliation":[{"name":"Bayerisches Landesamt f\u00fcr Gesundheit und Lebensmittelsicherheit, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter-Louis","family":"Plaumann","sequence":"additional","affiliation":[{"name":"Bayerisches Landesamt f\u00fcr Gesundheit und Lebensmittelsicherheit, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Ziegler","sequence":"additional","affiliation":[{"name":"Bayerisches Landesamt f\u00fcr Gesundheit und Lebensmittelsicherheit, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Knauer","sequence":"additional","affiliation":[{"name":"Bayerisches Landesamt f\u00fcr Gesundheit und Lebensmittelsicherheit, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Bogdan","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut\u2014Klinische Mikrobiologie, Immunologie und Hygiene, Universit\u00e4tsklinikum Erlangen, Friedrich-Alexander-Universit\u00e4t (FAU) Erlangen-N\u00fcrnberg, Erlangen, Germany"},{"name":"FAU Profilzentrum Immunmedizin, FAU Erlangen-N\u00fcrnberg, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Dudler","sequence":"additional","affiliation":[{"name":"Bayerisches Landesamt f\u00fcr Gesundheit und Lebensmittelsicherheit, Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","first-page":"2492","volume-title":"Mandell, Douglas and Bennett\u2019s principles and practice of infectious diseases","author":"Miller WR","year":"2020","unstructured":"Miller WR, Arias CA, Murray BE. 2020. 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