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Antibiotic failure is likely to be, at least in part, due to biofilm-dwelling\n                      <jats:italic toggle=\"yes\">B. pseudomallei,<\/jats:italic>\n                      and therefore, an improved understanding of how this pathogen regulates biofilm formation could reveal new opportunities for clinical intervention. The antimicrobial radical nitric oxide (NO) plays a key role in host immune defenses against bacteria, and the ability of\n                      <jats:italic toggle=\"yes\">B. pseudomallei<\/jats:italic>\n                      to sense and mitigate NO toxicity is vital for establishing infection. NO-sensing proteins (NosPs), which have recently emerged as key regulators of biofilm formation in many bacterial species, use a FIST domain to sense NO via a bound heme. We hypothesized that the NosP homolog in\n                      <jats:italic toggle=\"yes\">B. pseudomallei<\/jats:italic>\n                      would regulate biofilm formation and mediate NO-protective responses. We used [\u03b3-\n                      <jats:sup>32<\/jats:sup>\n                      P]ATP autophosphorylation assays to show that NosP of\n                      <jats:italic toggle=\"yes\">B. pseudomallei<\/jats:italic>\n                      controls the autophosphorylation rate of an associated histidine kinase protein (NosK) in an NO-dependent manner. NosK was found to phosphorylate a response regulator protein (NosR) with an HD-GYP output domain, which is associated with c-di-GMP signaling, therefore implicating NosP in modulating c-di-GMP-regulated phenotypes. Unmarked, in-frame deletion of either\n                      <jats:italic toggle=\"yes\">nosP<\/jats:italic>\n                      or\n                      <jats:italic toggle=\"yes\">nosK<\/jats:italic>\n                      caused significant changes in\n                      <jats:italic toggle=\"yes\">B. pseudomallei<\/jats:italic>\n                      biofilm formation and increased sensitivity to nitrosative stress, in addition to affecting other virulence traits such as growth and swimming motility. These results indicate that NosP and NosK signaling control a range of infection-relevant phenotypes and may serve as targets for novel therapeutic intervention.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Melioidosis is an emerging, potentially life-threatening infection caused by the bacterium\n                        <jats:italic toggle=\"yes\">Burkholderia pseudomallei<\/jats:italic>\n                        , killing ~89,000 people per year globally. Antibiotic therapy fails in ~10%\u201340% of cases, and hence, an improved understanding of the molecular mechanisms that control\n                        <jats:italic toggle=\"yes\">B. pseudomallei<\/jats:italic>\n                        virulence could reveal new approaches for improving melioidosis treatment. Biofilm formation and resistance to the antimicrobial radical NO are virulence traits that help bacteria establish infections. Here, we show that two proteins in\n                        <jats:italic toggle=\"yes\">B. pseudomallei<\/jats:italic>\n                        , NosP and NosK, work together to detect NO and regulate a suite of virulence traits, including NO resistance, biofilm formation, growth, and swimming motility. This work, therefore, improves our understanding of the molecular mechanisms that control infection-related phenotypes in\n                        <jats:italic toggle=\"yes\">B. pseudomallei<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00423-25","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T13:00:56Z","timestamp":1758114056000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["A nitric oxide-sensing two-component system regulates a range of infection-related phenotypes in\n                    <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8722-0564","authenticated-orcid":false,"given":"Matthew W.","family":"Scurlock","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03yghzc09","id-type":"ROR","asserted-by":"publisher"}],"name":"Biosciences, Faculty of Health and Life Sciences, University of Exeter","place":["Exeter, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1413-4961","authenticated-orcid":false,"given":"Stephen L.","family":"Michell","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03yghzc09","id-type":"ROR","asserted-by":"publisher"}],"name":"Biosciences, Faculty of Health and Life Sciences, University of Exeter","place":["Exeter, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7851-236X","authenticated-orcid":false,"given":"Steven L.","family":"Porter","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03yghzc09","id-type":"ROR","asserted-by":"publisher"}],"name":"Biosciences, Faculty of Health and Life Sciences, University of Exeter","place":["Exeter, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2015.8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.107"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20160063"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.12.011"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/fty023"},{"key":"e_1_3_3_7_2","first-page":"300","article-title":"Drug susceptibility and biofilm formation of Burkholderia pseudomallei in nutrient-limited condition","volume":"32","author":"Anutrakunchai C","year":"2015","unstructured":"Anutrakunchai C, Sermswan RW, Wongratanacheewin S, Puknun A, Taweechaisupapong S. 2015. 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