{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T23:08:57Z","timestamp":1790118537360,"version":"4.0.1"},"reference-count":80,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM137828"],"award-info":[{"award-number":["R35GM137828"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,9,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Incomplete antiseptic efficacy against potentially pathogenic microbial taxa places some patients at disproportionate risk for developing a surgical site infection. Laboratory models capable of interrogating the effects of antiseptics on the skin and its complex microbial communities are desperately needed to improve and better tailor antiseptic formulations. This work aims to establish an\n                      <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                      porcine skin model to explore the impact of topical antiseptics on complex skin microbial communities and superficial skin lipids. Microbiome samples were treated with propidium monoazide to selectively evaluate DNA from viable microorganisms. Bacterial abundances were assessed via viability-qPCR and quantitative culture. Viable community populations were evaluated with 16S rRNA gene sequencing. Epidermal biopsies were collected at multiple time points for lipidomic assessment via LC\/MS. The\n                      <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                      environment promoted shifts in porcine skin lipid composition and microbial communities over the experiment\u2019s duration. Compared to water-treated control skin, skin treated with the antiseptic chlorhexidine gluconate had significantly lower culturable counts and bioburden as determined by viability-qPCR. Compared to water-treated skin, viable microbial communities on CHG-treated skin displayed greater relative abundance of several gut-associated and Gram-negative bacterial taxa, including\n                      <jats:italic toggle=\"yes\">SMB53<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Turicibacter<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Proteus<\/jats:italic>\n                      . Collectively, these findings highlight the utility of an\n                      <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                      porcine skin system for interrogating the impacts of antimicrobial disruption on complex microbial ecosystems, and ultimately, for the future testing and development of improved antiseptic formulations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Broad-spectrum antiseptics are widely used to prevent surgical site infection and as wound cleansing agents. The impacts of such agents on beneficial microbes in the skin microbiome are understudied. Here, we describe an\n                      <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                      skin model to test the impacts of antiseptics or other topical agents on the healthy skin microbiome.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00441-25","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T12:00:15Z","timestamp":1756382415000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Establishing an\n                    <i>ex vivo<\/i>\n                    porcine skin model to investigate the effects of broad-spectrum antiseptic on viable skin microbial communities"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3102-8033","authenticated-orcid":false,"given":"E. 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J.","family":"Meudt","sequence":"additional","affiliation":[{"name":"Department of Animal & Dairy Sciences, University of Wisconsin","place":["Madison, USA"]},{"id":[{"id":"https:\/\/ror.org\/01y2jtd41","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Biomedical Swine Research & Innovation, University of Wisconsin School of Medicine and Public Health","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Shanmuganayagam","sequence":"additional","affiliation":[{"name":"Department of Animal & Dairy Sciences, University of Wisconsin","place":["Madison, USA"]},{"id":[{"id":"https:\/\/ror.org\/01y2jtd41","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Biomedical Swine Research & Innovation, University of Wisconsin School of Medicine and Public Health","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Huttenlocher","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y2jtd41","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health","place":["Madison, USA"]},{"id":[{"id":"https:\/\/ror.org\/01y2jtd41","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatrics, Division of Allergy, Immunology & Rheumatology, University of Wisconsin School of Medicine and Public Health","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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